FN Archimer Export Format
PT J
TI Influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (Sparus aurata) and intestinal contents in goldfish (Carassius auratus)
BT 
AF SILVA, Flavia Cristina de Paula
   NICOLI, Jacques Robert
   ZAMBONINO-INFANTE, Jose Luis
   KAUSHIK, Sadasivam
   GATESOUPE, François-Joel
AS 1:;2:;3:;4:;5:;
FF 1:;2:;3:PDG-RBE-PFOM-ARN;4:;5:;
C1 IFREMER, Ctr Brest, Lab ARN, F-29280 Plouzane, France.
   Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, Belo Horizonte, MG, Brazil.
   INRA, Nutr Metab Aquaculture UR1067, St Pee Sur Nivelle, France.
C2 IFREMER, FRANCE
   UNIV FED MINAS GERAIS, BRAZIL
   INRA, FRANCE
SI BREST
SE PDG-RBE-PFOM-ARN
   PDG-DOP-DCB-PFOM-ARN
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-int-hors-europe
   copubli-sud
IF 3.408
TC 93
UR https://archimer.ifremer.fr/doc/00040/15096/12440.pdf
LA English
DT Article
DE ;Vegetable meals;DGGE;microbiota;stomach;intestine;faeces
AB Fish intestinal microbiota changes with diet and this effect is of particular interest considering the increasing substitution of fish meal by plant protein sources. The objective of this work was to study the effects of partial substitution of fish meal with lupin and rapeseed meals on gut microbiota of the gilthead sea bream (Sparus aurata) and in goldfish (Carassius auratus). Faecal, gastrointestinal and intestinal contents were characterized by culture-based and molecular methods. Vibrionaceae was high in faeces and in the intestine of sea bream, while a more diverse microbiota was retrieved from the stomach, where Bacillales and Flavobacteriaceae appeared to be influenced by the diet. PCR-DGGE profiles revealed high diversity of the microbiota transiting in the sea bream digestive tract, with a shift between gastric and intestinal communities, specially in the group fed with lupin meal. The goldfish was different, with predominance of Aeromonas spp., Shewanella putrefaciens and Staphylococcus spp. among the aerotolerant cultivable bacteria. The culture-independent methods revealed the presence of anaerobes like Cetobacterium somerae, and that of Vibrio spp., likely in a viable but non-cultivable state. There was a trend towards decreasing diversity in goldfish microbiota with the partial substitution by lupin, which seemed to inhibit some taxa.
PY 2011
PD NOV
SO Fems Microbiology Ecology
SN 0168-6496
PU Wiley-blackwell
VL 78
IS 2
UT 000296849000010
BP 285
EP 296
DI 10.1111/j.1574-6941.2011.01155.x
ID 15096
ER

EF