Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster, Pinctada margaritifera
|Author(s)||Hui Belinda1, Vonau Vincent1, Moriceau Jacques1, Tetumu Roger1, Vanaa Vincent1, Demoy-Schneider Marina2, Suquet Marc3, Le Moullac Gilles1|
|Affiliation(s)||1 : IFREMER, Lab Domesticat Huitre Perliere, Ctr Pacifique, Taravao 98719, Tahiti, Fr Polynesia
2 : Univ Polynesie Francaise, CNRS, UMR EA4239, Lab Biodivers Terr & Marine, Faaa 98702, Tahiti, Fr Polynesia
3 : IFREMER, UMR Physiol & Ecophysiol Mollusques Marins 100, F-29840 Argenton En Landunvez, France
|Source||Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2011-04 , Vol. 24 , N. 2 , P. 219-223|
|WOS© Times Cited||17|
|Keyword(s)||Cryopreservation, Spermatozoa, Fertility, Larval rearing, Pearl oyster, Pinctada margaritifera|
|Abstract||Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry.
Spermatozoa were cryopreserved with CPA 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23 % lower than that of fresh sperm. When using thawed sperm, therefore, a higher sperm/egg ratio of 100 000:1 was required to reach 80 % oocyte fertilization, compared with 100:1 for fresh sperm. Nevertheless, this first demonstration of cryopreserved sperm fertility in black pearl oyster confirms the hatchery applicability of the cryopreservation technique defined here. Monitoring for larval viability during the first 23 days of life revealed no significant differences between the progeny produced with cryopreserved sperm and that produced using fresh sperm.