FN Archimer Export Format
PT J
TI Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster, Pinctada margaritifera
BT 
AF HUI, Belinda
   VONAU, Vincent
   MORICEAU, Jacques
   TETUMU, Roger
   VANAA, Vincent
   DEMOY-SCHNEIDER, Marina
   SUQUET, Marc
   LE MOULLAC, Gilles
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:3;8:1;
FF 1:PDG-DOP-DCOP-AQUAPOL-LDHP;2:PDG-RBE-RMPF-EP;3:PDG-RBE-PFOM-ARN;4:PDG-RBE-RMPF-EP;5:PDG-RBE-RMPF-EP;6:;7:PDG-RBE-PFOM-PI;8:PDG-RBE-RMPF-EP;
C1 IFREMER, Lab Domesticat Huitre Perliere, Ctr Pacifique, Taravao 98719, Tahiti, Fr Polynesia
   Univ Polynesie Francaise, CNRS, UMR EA4239, Lab Biodivers Terr & Marine, Faaa 98702, Tahiti, Fr Polynesia
   IFREMER, UMR Physiol & Ecophysiol Mollusques Marins 100, F-29840 Argenton En Landunvez, France
C2 IFREMER, FRANCE
   UNIV POLYNESIE FRANCAISE, FRANCE
   IFREMER, FRANCE
SI TAHITI
   BREST
   ARGENTON
SE PDG-DOP-DCOP-AQUAPOL-LDHP
   PDG-RBE-RMPF-EP
   PDG-RBE-PFOM-ARN
   PDG-RBE-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.152
TC 18
UR https://archimer.ifremer.fr/doc/00040/15098/12446.pdf
LA English
DT Article
DE ;Cryopreservation;Spermatozoa;Fertility;Larval rearing;Pearl oyster;Pinctada margaritifera
AB Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry.  Spermatozoa were cryopreserved with CPA 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23 % lower than that of fresh sperm. When using thawed sperm, therefore, a higher sperm/egg ratio of 100 000:1 was required to reach 80 % oocyte fertilization, compared with 100:1 for fresh sperm. Nevertheless, this first demonstration of cryopreserved sperm fertility in black pearl oyster confirms the hatchery applicability of the cryopreservation technique defined here. Monitoring for larval viability during the first 23 days of life revealed no significant differences between the progeny produced with cryopreserved sperm and that produced using fresh sperm.
PY 2011
PD APR
SO Aquatic Living Resources
SN 0990-7440
PU Edp Sciences S A
VL 24
IS 2
UT 000293593900014
BP 219
EP 223
DI 10.1051/alr/2011117
ID 15098
ER

EF