FN Archimer Export Format
PT J
TI The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas
BT 
AF SEGUINEAU, Catherine
   RACOTTA, Ilie
   PALACIOS, Elena
   DELAPORTE, Maryse
   MOAL, Jeanne
   SOUDANT, Philippe
AS 1:1;2:2,3;3:3,4;4:2;5:2;6:4;
FF 1:;2:;3:;4:PDG-DOP-DCB-PFOM-PI;5:PDG-RBE-PFOM-PI;6:;
C1 Université de Bretagne Occidentale, Brest, France et UMR 100 Physiologie et Ecophysiologie des Mollusques Marins, Centre IFREMER de Brest, BP70, 29280 Plouzané, France
   UMR 100 Physiologie et Ecophysiologie des Mollusques Marins, Centre IFREMER de Brest, BP70, 29280 Plouzané, France
   Programa de Acuicultura, Centro de Investigaciones Biológicas del Noroeste, Apdo. Postal 128, La Paz B.C.S. 23090, Mexico
   Laboratoire des Sciences de l'Environnement Marin, UMR 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Technopôle Brest-Iroise, 29280 Plouzané, France
C2 UBO, FRANCE
   IFREMER, FRANCE
   CIBNOR, MEXICO
   UBO, FRANCE
SI BREST
SE PDG-RBE-PFOM-PI
   PDG-DOP-DCB-PFOM-PI
   EXTERIEUR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 2.235
TC 8
UR https://archimer.ifremer.fr/doc/00040/15103/12785.pdf
LA English
DT Article
DE ;Prostaglandins;n-6 unsaturated fatty acids;Lipid peroxidation;Antioxidants;ROS production
AB In a previous study, dietary supplementation with arachidonic acid (ARA) to oysters Crassostrea gigas increased haemocyte numbers, phagocytosis, and production of reactive oxygen species level (ROS) by haemocytes (Delaporte et al., 2006). To assess if the observed stimulation of these cellular responses resulted from changes of ARA-related prostaglandin (PG) production, we analysed prostaglandin E2 metabolite (PGEM) content on the same oysters fed three levels of ARA. Dietary supply of polyunsaturated fatty acids (PUFA) could also induce an oxidative stress that could similarly increase cellular responses; therefore, two indicators of oxidative stress were analysed: peroxidation level and antioxidant defence status. Together the observed positive correlation between ARA and PGEM levels and the absence of lipid peroxidation and antioxidant activity changes supports the hypothesis of an immune stimulation via PG synthesis. Although ARA proportion in oyster tissues increased by up to 7-fold in response to ARA dietary supplementation, peroxidation index did not change because of a compensatory decrease in n-3 fatty acid proportion, mainly 22:6n-3. To further confirm the involvement of PG in the changes of haemocyte count, phagocytosis and ROS production upon ARA supplementation, it would be interesting to test cyclooxygenase and lipooxygenase inhibitors in similar experiments.
PY 2011
PD SEP
SO Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
SN 1095-6433
PU Elsevier
VL 160
IS 1
UT 000293486200012
BP 87
EP 93
DI 10.1016/j.cbpa.2011.05.011
ID 15103
ER

EF