TY - JOUR T1 - Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts A1 - Pasquet,Virginie A1 - Morisset,Perrine A1 - Ihammouine,Said A1 - Chepied,Amandine A1 - Aumailley,Lucie A1 - Berard,Jean-Baptiste A1 - Serive,Benoit A1 - Kaas,Raymond A1 - Lanneluc,Isabelle A1 - Thiery,Valerie A1 - Lafferriere,Mathieu A1 - Piot,Jean-Marie A1 - Patrice,Thierry A1 - Cadoret,Jean-Paul A1 - Picot,Laurent AD - Univ La Rochelle, UMR CNRS LIENSs 6250, F-17042 La Rochelle, France AD - IFREMER Ctr Nantes, IFREMER Lab PBA, F-44311 Nantes, France AD - CHU Nantes, Dept LASER, F-44093 Nantes, France UR - https://archimer.ifremer.fr/doc/00040/15142/ DO - 10.3390/md9050819 KW - pigments KW - microalgae KW - Dunaliella KW - violaxanthin KW - carotenoid KW - cancer KW - apoptosis N2 - Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 mu g.mL(-1) (0.17 mu M). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 mu g.mL(-1) (13.3 mu M) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 mu g.mL(-1) (66.7 mu M). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained. Y1 - 2011/05 PB - Mdpi Ag JF - Marine Drugs SN - 1660-3397 VL - 9 IS - 5 SP - 819 EP - 831 ID - 15142 ER -