FN Archimer Export Format
PT J
TI Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts
BT 
AF PASQUET, Virginie
   MORISSET, Perrine
   IHAMMOUINE, Said
   CHEPIED, Amandine
   AUMAILLEY, Lucie
   BERARD, Jean-Baptiste
   SERIVE, Benoit
   KAAS, Raymond
   LANNELUC, Isabelle
   THIERY, Valerie
   LAFFERRIERE, Mathieu
   PIOT, Jean-Marie
   PATRICE, Thierry
   CADORET, Jean-Paul
   PICOT, Laurent
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:2;8:2;9:1;10:1;11:1;12:1;13:3;14:2;15:1;
FF 1:;2:;3:;4:;5:;6:PDG-RBE-BRM-PBA;7:PDG-RBE-BRM-PBA;8:PDG-RBE-BRM-PBA;9:;10:;11:;12:;13:;14:PDG-RBE-BRM-PBA;15:;
C1 Univ La Rochelle, UMR CNRS LIENSs 6250, F-17042 La Rochelle, France
   IFREMER Ctr Nantes, IFREMER Lab PBA, F-44311 Nantes, France
   CHU Nantes, Dept LASER, F-44093 Nantes, France
C2 UNIV LA ROCHELLE, FRANCE
   IFREMER, FRANCE
   CHU NANTES, FRANCE
SI NANTES
SE PDG-RBE-BRM-PBA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.854
TC 73
UR https://archimer.ifremer.fr/doc/00040/15142/12575.pdf
LA English
DT Article
DE ;pigments;microalgae;Dunaliella;violaxanthin;carotenoid;cancer;apoptosis
AB Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 mu g.mL(-1) (0.17 mu M). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 mu g.mL(-1) (13.3 mu M) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 mu g.mL(-1) (66.7 mu M). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained.
PY 2011
PD MAY
SO Marine Drugs
SN 1660-3397
PU Mdpi Ag
VL 9
IS 5
UT 000292632500010
BP 819
EP 831
DI 10.3390/md9050819
ID 15142
ER

EF