A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas

Type Article
Date 2011-10
Language English
Author(s) Corporeau Charlotte1, Groisillier Agnes2, Jeudy Alexandra2, Barbeyron Tristan2, Fleury ElodieORCID1, Fabioux Caroline3, Czjzek Mirjam2, Huvet ArnaudORCID1
Affiliation(s) 1 : IFREMER, Ctr Brest, PE2M, UMR 100, F-29280 Plouzane, France
2 : Univ Paris 06, Biol Stn, CNRS UPMC, LIA DIAMS,UMR Vegetaux Marines & Biomol 7139, F-29682 Roscoff, France
3 : Univ Bretagne Occidentale, CNRS, LEMAR, IUEM,UMR 6539, F-29280 Plouzane, France
Source Marine Biotechnology (1436-2228) (Springer), 2011-10 , Vol. 13 , N. 5 , P. 971-980
DOI 10.1007/s10126-010-9361-4
WOS© Times Cited 10
Keyword(s) Transforming growth factor-beta, Bacterial expression, In vivo antibody inhibition, Reproduction, Crassostrea gigas, Marine bivalve
Abstract The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a rapid bacterial production system to obtain recombinant og TGF-beta protein, and we demonstrate that og TGF-beta is processed by furin to a mature form of the protein. This mature form can be detected in vivo in the gonad. Functional inhibition of mature og TGF-beta in the gonad was conducted by inactivation of the protein using injection of antibodies. We show that inhibition of og TGF-beta function tends to reduce gonadic area. We conclude that mature og TGF-beta probably functions as an activator of germ cells development in oyster.
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Corporeau Charlotte, Groisillier Agnes, Jeudy Alexandra, Barbeyron Tristan, Fleury Elodie, Fabioux Caroline, Czjzek Mirjam, Huvet Arnaud (2011). A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas. Marine Biotechnology, 13(5), 971-980. Publisher's official version : https://doi.org/10.1007/s10126-010-9361-4 , Open Access version : https://archimer.ifremer.fr/doc/00042/15371/