FN Archimer Export Format
PT J
TI A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas
BT 
AF CORPOREAU, Charlotte
   GROISILLIER, Agnes
   JEUDY, Alexandra
   BARBEYRON, Tristan
   FLEURY, Elodie
   FABIOUX, Caroline
   CZJZEK, Mirjam
   HUVET, Arnaud
AS 1:1;2:2;3:2;4:2;5:1;6:3;7:2;8:1;
FF 1:PDG-RBE-PFOM-PI;2:;3:;4:;5:PDG-ODE-LER-LERMPL;6:;7:;8:PDG-RBE-PFOM-PI;
C1 IFREMER, Ctr Brest, PE2M, UMR 100, F-29280 Plouzane, France
   Univ Paris 06, Biol Stn, CNRS UPMC, LIA DIAMS,UMR Vegetaux Marines & Biomol 7139, F-29682 Roscoff, France
   Univ Bretagne Occidentale, CNRS, LEMAR, IUEM,UMR 6539, F-29280 Plouzane, France
C2 IFREMER, FRANCE
   UNIV PARIS 06, FRANCE
   UBO, FRANCE
SI BREST
   LA TRINITE
SE PDG-RBE-PFOM-PI
   PDG-ODE-LER-LERMPL
   IUEM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.43
TC 10
UR https://archimer.ifremer.fr/doc/00042/15371/13438.pdf
LA English
DT Article
DE ;Transforming growth factor-beta;Bacterial expression;In vivo antibody inhibition;Reproduction;Crassostrea gigas;Marine bivalve
AB The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a rapid bacterial production system to obtain recombinant og TGF-beta protein, and we demonstrate that og TGF-beta is processed by furin to a mature form of the protein. This mature form can be detected in vivo in the gonad. Functional inhibition of mature og TGF-beta in the gonad was conducted by inactivation of the protein using injection of antibodies. We show that inhibition of og TGF-beta function tends to reduce gonadic area. We conclude that mature og TGF-beta probably functions as an activator of germ cells development in oyster.
PY 2011
PD OCT
SO Marine Biotechnology
SN 1436-2228
PU Springer
VL 13
IS 5
UT 000293970600016
BP 971
EP 980
DI 10.1007/s10126-010-9361-4
ID 15371
ER

EF