FN Archimer Export Format PT J TI Calicivirus Removal in a Membrane Bioreactor Wastewater Treatment Plant BT AF SIMA, Laura C. SCHAEFFER, Julien LE SAUX, Jean-Claude PARNAUDEAU, Sylvain ELIMELECH, Menachem LE GUYADER, Soizick AS 1:2;2:1;3:1;4:1;5:2;6:1; FF 1:;2:PDG-RBE-EMP-MIC;3:PDG-RBE-EMP-MIC;4:PDG-RBE-EMP-MIC;5:;6:PDG-RBE-EMP-MIC; C1 IFREMER, Lab Microbiol LNR, F-44311 Nantes 03, France Yale Univ, Dept Chem Engn, Environm Engn Program, New Haven, CT 06520 USA C2 IFREMER, FRANCE UNIV YALE, USA SI NANTES BREST SE PDG-RBE-EMP-MIC IN WOS Ifremer jusqu'en 2018 copubli-int-hors-europe IF 3.829 TC 61 UR https://archimer.ifremer.fr/doc/00043/15378/12810.pdf LA English DT Article AB To evaluate membrane bioreactor wastewater treatment virus removal, a study was conducted in southwest France. Samples collected from plant influent, an aeration basin, membrane effluent, solid sludge, and effluent biweekly from October 2009 to June 2010 were analyzed for calicivirus (norovirus and sapovirus) by real-time reverse transcription-PCR (RT-PCR) using extraction controls to perform quantification. Adenovirus and Escherichia coli also were analyzed to compare removal efficiencies. In the influent, sapovirus was always present, while the norovirus concentration varied temporally, with the highest concentration being detected from February to May. All three human norovirus genogroups (GI, GII, and GIV) were detected in effluent, but GIV was never detected in effluent; GI and GII were detected in 50% of the samples but at low concentrations. In the effluent, sapovirus was identified only once. An adenovirus titer showing temporal variation in influent samples was identified only twice in effluent. E. coli was always below the limit of detection in the effluent. Overall, the removal of calicivirus varied from 3.3 to greater than 6.8 log units, with no difference between the two main genogroups. Our results also demonstrated that the viruses are blocked by the membrane in the treatment plant and are removed from the plant as solid sludge. PY 2011 PD AUG SO Applied And Environmental Microbiology SN 0099-2240 PU Amer Soc Microbiology VL 77 IS 15 UT 000293224500012 BP 5170 EP 5177 DI 10.1128/AEM.00583-11 ID 15378 ER EF