FN Archimer Export Format
PT J
TI Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1
BT 
AF RENAULT, Tristan
   FAURY, Nicole
   BARBOSA SOLOMIEU, Valérie
   MOREAU, Kevin
AS 1:1;2:1;3:1;4:1;
FF 1:PDG-RBE-AGSAE-LGP;2:PDG-RBE-AGSAE-LGP;3:;4:PDG-DOP-DCN-AGSAE-LGP;
C1 Ifremer, Laboratoire de Génétique et Pathologie, 17390 La Tremblade, France
C2 IFREMER, FRANCE
SI LA TREMBLADE
SE PDG-RBE-AGSAE-LGP
   PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
IF 3.268
TC 125
UR https://archimer.ifremer.fr/doc/00043/15466/12843.pdf
LA English
DT Article
DE ;Pacific cupped oyster;Crassostrea gigas;OsHV-1;Immunity;Gene expression;Multicopper oxidase;Macrophage expressed protein;Ig cell adhesion molecule;Interferon-induced protein 44;Virus RNA
AB Virus-induced genes were identified using suppression subtractive hybridisation (SSH) from Pacific cupped oyster, Crassostrea gigas, haemocytes challenged by OsHV-1. A total of 304 clones from SSH forward library were sequenced. Among these sequences, some homologues corresponded to (i) immune related genes (macrophage express protein, IK cytokine, interferon-induced protein 44 or multicopper oxidase), (ii) apoptosis related genes (Bcl-2) and (iii) cell signalling and virus receptor genes (glypican). Molecular characterization and phylogenic analysis of 3 immune-related genes (macrophage expressed protein, multicopper oxidase and immunoglobulin domain cell adhesion molecule) were performed. Finally, quantitative PCR revealed significant changes in the expression of immune related genes (multicopper oxidase, macrophage expressed protein, myeloid differentiation factor 88 and interferon-induced protein 44) in oysters experimentally challenged with OsHV-1.  These findings provide a first basis for studying the role of innate immunity in response to viruses in bivalves and identified genes may serve as markers of interest in breeding programs in order to obtain selected oysters presenting OsHV-1 resistance.
PY 2011
PD JUN
SO Developmental & Comparative Immunology
SN 0145-305X
PU Elsevier
VL 35
IS 7
UT 000291071900003
BP 725
EP 735
DI 10.1016/j.dci.2011.02.004
ID 15466
ER

EF