FN Archimer Export Format PT J TI Disseminated neoplasia in cockles Cerastoderma edule: ultrastructural characterisation and effects on haemolymph cell parameters BT AF DIAZ, Seila RENAULT, Tristan VILLALBA, Antonio CARBALLAL, María Jesús AS 1:1;2:2;3:1;4:1; FF 1:;2:PDG-RBE-AGSAE-LGP;3:;4:; C1 Ctr Invest Marinas, Xunta De Galicia 36620, Vilanova Arousa, Spain IFREMER, Lab Genet & Pathol, F-17390 Ronce Les Bains, La Tremblade, France C2 CIMA, SPAIN IFREMER, FRANCE SI LA TREMBLADE SE PDG-RBE-AGSAE-LGP IN WOS Ifremer jusqu'en 2018 copubli-europe IF 2.201 TC 12 UR https://archimer.ifremer.fr/doc/00043/15468/12846.pdf https://archimer.ifremer.fr/doc/00043/15468/12847.pdf LA English DT Article AB Disseminated neoplasia (DN) has been detected in cockles from various beds in Galicia (NW Spain). A study was performed to characterise cockle neoplastic cell ultrastructure and to evaluate the effect of this disease at different severity stages on various haemolymph cell parameters. Examination of cockle neoplastic cells with transmission electron microscopy (TEM) showed round shapes and a lack of pseudopods, a high nucleus:cytoplasm diameter ratio, Golgi complexes, abundant mitochondria, ribosomes, and numerous endoplasmic reticulum tubes and electron-lucent vesicles. Various haemolymph cell parameters (cell mortality, non-specific esterase and lysosome biovolume, reactive oxygen intermediates [ROI] production, phagocytosis ability, intracellular Ca2+ and actin levels) were compared between DN severity categories by flow cytometry; haemocyte mortality, non-specific esterase activities and lysosome biovolume were found to be higher with increasing DN severity. The phagocytic ability of neoplastic cells was sharply reduced with regard to haemocytes. The cytoplasmic-free Ca2+ level was higher and actin content lower in haemolymph cells of diseased cockles compared to unaffected ones. A significant increase in ROI production was detected in later stages of disease progression. PY 2011 PD SEP SO Diseases Of Aquatic Organisms SN 0177-5103 VL 96 IS 2 UT 000294732900008 BP 157 EP 167 DI 10.3354/dao02384 ID 15468 ER EF