TY - JOUR T1 - Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range A1 - Massa,Sonia I. A1 - Pearson,Gareth A. A1 - Aires,Tania A1 - Kube,Michael A1 - Olsen,Jeanine L. A1 - Reinhardt,Richard A1 - Serrao,Ester A. A1 - Arnaud-Haond,Sophie AD - IFREMER, Ctr Brest, F-29280 Plouzane, France AD - Univ Groningen, Dept Marine Benth Ecol & Evolut, Ctr Ecol & Evolutionary Studies, Ctr Biol, NL-9750 AA Haren, Netherlands AD - Max Planck Inst Mol Genet, D-14195 Berlin, Germany AD - Univ Algarve, CCMAR CIMAR, P-8005139 Faro, Portugal UR - https://doi.org/10.1016/j.margen.2011.04.003 DO - 10.1016/j.margen.2011.04.003 KW - EST library KW - High temperature stress KW - Abiotic stress response KW - Climate change N2 - Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera midi during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30 min, 2 h, 4 h and 24 h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as 'molecular chaperones' and were over-expressed following heat-shock, while 12 (23.5%) were 'photosynthesis TUGs' generally under-expressed in heat-shocked plants. A time course analysis of expression showed a rapid increase in expression of the molecular chaperone class, most of which were heat-shock proteins; which increased from 2 sequence reads in the control library to almost 230 in the 30 min AHS library, followed by a slow decrease during further recovery. In contrast, 'photosynthesis TUGs' were under-expressed 30 min AHS compared with the control library, and declined progressively with recovery time in the stress libraries, with a total of 29 sequence reads 24 h AHS, compared with 125 in the control. A total of 4734 TUGs were screened for EST-Single Sequence Repeats (EST-SSRs) and 86 microsatellites were identified. (C) 2011 Elsevier B.V. All rights reserved. Y1 - 2011/09 PB - Elsevier Science Bv JF - Marine Genomics SN - 1874-7787 VL - 4 IS - 3 SP - 181 EP - 188 ID - 15559 ER -