FN Archimer Export Format
PT J
TI Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range
BT 
AF MASSA, Sonia I.
   PEARSON, Gareth A.
   AIRES, Tania
   KUBE, Michael
   OLSEN, Jeanine L.
   REINHARDT, Richard
   SERRAO, Ester A.
   ARNAUD-HAOND, Sophie
AS 1:;2:;3:;4:;5:;6:;7:;8:;
FF 1:;2:;3:;4:;5:;6:;7:;8:PDG-DOP-DCB-EEP-LEP;
C1 IFREMER, Ctr Brest, F-29280 Plouzane, France
   Univ Groningen, Dept Marine Benth Ecol & Evolut, Ctr Ecol & Evolutionary Studies, Ctr Biol, NL-9750 AA Haren, Netherlands
   Max Planck Inst Mol Genet, D-14195 Berlin, Germany
   Univ Algarve, CCMAR CIMAR, P-8005139 Faro, Portugal
C2 IFREMER, FRANCE
   UNIV GRONINGEN, NETHERLANDS
   INST MAX PLANCK (MOL GENET), GERMANY
   UNIV ALGARVE, PORTUGAL
SI BREST
SE PDG-REM-EEP-LEP
   PDG-DOP-DCB-EEP-LEP
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 1.551
TC 18
UR https://archimer.ifremer.fr/doc/00044/15559/13326.pdf
LA English
DT Article
DE ;EST library;High temperature stress;Abiotic stress response;Climate change
AB Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera midi during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30 min, 2 h, 4 h and 24 h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as 'molecular chaperones' and were over-expressed following heat-shock, while 12 (23.5%) were 'photosynthesis TUGs' generally under-expressed in heat-shocked plants. A time course analysis of expression showed a rapid increase in expression of the molecular chaperone class, most of which were heat-shock proteins; which increased from 2 sequence reads in the control library to almost 230 in the 30 min AHS library, followed by a slow decrease during further recovery. In contrast, 'photosynthesis TUGs' were under-expressed 30 min AHS compared with the control library, and declined progressively with recovery time in the stress libraries, with a total of 29 sequence reads 24 h AHS, compared with 125 in the control. A total of 4734 TUGs were screened for EST-Single Sequence Repeats (EST-SSRs) and 86 microsatellites were identified. (C) 2011 Elsevier B.V. All rights reserved.
PY 2011
PD SEP
SO Marine Genomics
SN 1874-7787
PU Elsevier Science Bv
VL 4
IS 3
UT 000294985500007
BP 181
EP 188
DI 10.1016/j.margen.2011.04.003
ID 15559
ER

EF