FN Archimer Export Format PT J TI Alexandrium ostenfeldii growth and spirolide production in batch culture and photobioreactor BT AF MEDHIOUB, Walid SECHET, Veronique TRUQUET, Philippe BARDOUIL, Michele AMZIL, Zouher LASSUS, Patrick SOUDANT, Philippe AS 1:1,2,3;2:2;3:2;4:2;5:2;6:2;7:1; FF 1:;2:PDG-RBE-EMP-PHYC;3:PDG-RBE-EMP-PHYC;4:PDG-RBE-EMP-PHYC;5:PDG-RBE-EMP-PHYC;6:PDG-RBE-EMP-PHYC;7:; C1 Univ Bretagne Occidentale, IUEM, LEMAR UMR 6539, F-29280 Plouzane, France. IFREMER, Lab Phycotoxines, F-44311 Nantes 3, France. INSTM, Lab Aquaculture, Monastir 5000, Tunisia. C2 UBO, FRANCE IFREMER, FRANCE INSTM, TUNISIA SI MONTPELLIER NANTES BREST SE PDG-RBE-BOME-LALR PDG-RBE-EMP-PHYC EXTERIEUR IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france copubli-int-hors-europe copubli-sud IF 3.083 TC 12 UR https://archimer.ifremer.fr/doc/00049/16050/15784.pdf LA English DT Article DE ;Alexandrium ostenfeldii;Batch;Growth;Photobioreactor;Spirolide production AB Growth and spirolide production of the toxic dinoflagellate Alexandrium ostenfeldii (Danish strain CCMP1773) were studied in batch culture and a photobioreactor (continuous cultures). First, batch cultures were grown in 450 mL flasks without aeration and under varying conditions of temperature (16 and 22 degrees C) and culture medium (L1, f/2 and L1 with addition of soil extract). Second, cultures were grown at 16 degrees C in 8 L aerated flat-bottomed vessels using L1 with soil extract as culture medium. Finally, continuous cultures in a photobioreactor were conducted at 18 degrees C in L1 with soil extract; pH was maintained at 8.5 and continuous stirring was applied. This study showed that A. ostenfeldii growth was significantly affected by temperature. At the end of the exponential phase, maximum cell concentration and cell diameter were significantly higher at 16 degrees C than at 22 C. In batch culture, maximum spirolide quota per cell (approx. 5 pg SPX 13-desMeC eq cell(-1)) was detected during lag phase for all conditions used. Spirolide quota per cell was negatively and significantly correlated to cell concentration according to the following equation: y = 4013.9x(-0.858). Temperature and culture medium affected the spirolide profile which was characterized by the dominance of 13,19-didesMeC (29-46%), followed by SPX-D (21-28%), 13-desMeC (21-23%), and 13-desMeD (17-21%). Stable growth of A. ostenfeldii was maintained in a photobioreactor over two months, with maximum cell concentration of 7 x 10(4) cells mL(-1). As in batch culture, maximum spirolide cell quota was found in lag phase and then decreased significantly throughout the exponential phase. Spirolide cell quota was negatively and significantly correlated to cell concentration according to the equation: y = 12,858x(-0.8986). In photobioreactor, spirolide profile was characterized by higher proportion of 13,19-didesMeC (60-87%) and lower proportions of SPX-D (3-12%) and 13-desMeD (1.6-10%) as compared to batch culture. (C) 2011 Elsevier B.V. All rights reserved. PY 2011 PD SEP SO Harmful Algae SN 1568-9883 PU Elsevier Science Bv VL 10 IS 6 UT 000296112700029 BP 794 EP 803 DI 10.1016/j.hal.2011.06.012 ID 16050 ER EF