FN Archimer Export Format PT J TI Feeding the pearl oyster Pinctada margaritifera during reproductive conditioning BT AF LE MOULLAC, Gilles SOYEZ, Claude SHAM-KOUA, Manaarii LEVY, Peva MORICEAU, Jacques VONAU, Vincent MAIHOTA, Mayalen COCHARD, Jean-Claude AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;8:2; FF 1:PDG-RBE-RMPF-EP;2:PDG-RBE-RMPF-EP;3:PDG-RBE-RMPF-EP;4:PDG-RBE-RMPF-EP;5:PDG-RBE-PFOM-ARN;6:PDG-RBE-RMPF-EP;7:PDG-RBE-RMPF-EP;8:PDG-ODE-LER; C1 IFREMER, Ctr Pacifique, Lab Ecosyst Perlicole, Taravao 98719, Fr Polynesia. IFREMER, Ctr Brest, Dept Environm Littoral & Ressources aquacoles, F-29280 Plouzane, France. C2 IFREMER, FRANCE IFREMER, FRANCE SI TAHITI BREST SE PDG-RBE-RMPF-EP PDG-RBE-PFOM-ARN PDG-ODE-LER IN WOS Ifremer jusqu'en 2018 IF 1.32 TC 14 UR https://archimer.ifremer.fr/doc/00050/16085/13562.pdf LA English DT Article DE ;Pinctada margaritifera;food level;ingestion rate;reproduction;gametogenesis AB This study aimed to model the food intake of P. margaritifera in order to examine the relationship between food level and reproductive activity. The effect of microalgae concentration on ingestion rate and assimilation efficiency was studied over a broad concentration range, using a mixture of Isochrysis galbana and Chaetoceros gracilis. Reproductive effort was assessed using three microalgae concentrations of 0.5, 7 and 18 cell μL-1. Reproductive status was assessed by gonad development index (GDI) – the ratio of the gonad surface to the visceral mass surface – and histological analysis of the gonad based on the presence (continuous or discontinuous) or the absence of gonial cells (GC). Ingestion is a saturating function of seston concentration for bivalves modeled with an adapted Michaelis- Menten function. The maximum ingestion rate of P. margaritifera adults was 193.50 106 cell.h-1.g-1 dw and the half saturation coefficient was 15 cell.μL-1. The concentration of 18 cell μL-1, supplied for 45 days induced a significantly higher GDI than the other treatments. GC decreased significantly and even stopped when pearl oysters were under-fed, suggesting that the mitotic process of the germinal stem cells was altered. Differentiation of germinal stem cells, therefore, appears to be controlled by food availability. PY 2013 PD FEB SO Aquaculture Research SN 1355-557X PU Wiley-blackwell VL 44 IS 3 UT 000314566200010 BP 404 EP 411 DI 10.1111/j.1365-2109.2011.03045.x ID 16085 ER EF