FN Archimer Export Format
PT J
TI Physiological and biochemical changes associated with massive mortality events occurring in larvae of American oyster (Crassostrea virginica)
BT 
AF GENARD, Bertrand
   PERNET, Fabrice
   LEMARCHAND, Karine
   BOUDRY, Pierre
   MORAGA, Dario
   TREMBLAY, Rejean
AS 1:1;2:2,3;3:1;4:4;5:5;6:1;
FF 1:;2:PDG-ODE-LER-LERLR;3:;4:PDG-RBE-PFOM-PI;5:;6:;
C1 Inst Rech Zones Cotieres, Shippegan, NB E8S 2L7, Canada.
   Univ Quebec, Inst Sci Mer, Rimouski, PQ G5L 3A1, Canada.
   IFREMER, Lab Environm Ressources Languedoc Roussillon, F-34203 Sete, France.
   IFREMER, UMR Physiol & Ecophysiol Mollusques Marins M100, F-29280 Plouzane, France.
   Univ Bretagne Occidentale, Inst Univ Europeen Mer, Lab Sci Environm Marin, F-29280 Plouzane, France.
C2 IRZC, CANADA
   UNIV QUEBEC, CANADA
   IFREMER, FRANCE
   IFREMER, FRANCE
   UBO, FRANCE
SI SETE
   BREST
SE PDG-ODE-LER-LERLR
   PDG-RBE-PFOM-PI
   IUEM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 1.152
TC 23
UR https://archimer.ifremer.fr/doc/00051/16231/13753.pdf
LA English
DT Article
DE ;Massive mortality;Larvae;Microbial environment;Lipid;Fatty acids;Energy metabolism;Oxidative stress;Antioxidant enzymes;Crassostrea virginica
AB In this paper, biochemical and physiological analyses were used to characterize changes associated with mortality event occurred during veliger development of American oyster, Crassostrea virginica. Biochemical analyses included the evaluation of lipid classes, fatty acid composition and total protein content. Larval physiology was evaluated by studying feeding activity, enzymes related to energy metabolism, oxidative stress levels and enzymatic antioxidant defenses. These analyses were complemented by bacterial community analyses as well as by measuring larval oyster performance. We observed that mortality events coincided with (1) strong changes in the surrounding bacterial community; (2) a progressive decrease in feeding activity; (3) higher levels of some lipid classes (free fatty acids, diglycerides, and acetone mobile phospholipids); (4) lower levels of phospholipids and protein; (5) higher contents of non-methylene interrupted dienoic fatty acids (22:2 NMI); (6) a decrease in energy metabolism activity (citrate synthase and cytochrome oxidase activities); (7) a higher oxidative stress (lipid peroxidation level); and (8) an activation of antioxidant defences before mortality (glutathione peroxidase and superoxide dismutase). We hypothesized that mortality emergence was related to higher energy consumption coupled with the progressive decline in feeding activity, lowered energy reserves and a decrease in energy metabolism activity. Thus, the low energy availability limited the efficiency of antioxidant defenses, resulting in a higher oxidative stress.
PY 2011
PD JUN
SO Aquatic Living Resources
SN 0990-7440
PU Edp Sciences S A
VL 24
IS 3
UT 000296576800004
BP 247
EP 260
DI 10.1051/alr/2011114
ID 16231
ER

EF