TY - JOUR T1 - Characterisation of the spoilage microbiota in raw salmon (Salmo salar) steaks stored under vacuum or modified atmosphere packaging combining conventional methods and PCR–TTGE A1 - Mace,Sabrina A1 - Cornet,Josiane A1 - Chevalier,Frederique A1 - Cardinal,Mireille A1 - Pilet,Marie-France A1 - Dousset,Xavier A1 - Joffraud,Jean-Jacques AD - IFREMER, Lab Sci & Technol Biomasse Marine, F-44311 Nantes 3, France. AD - Univ Nantes, Oniris, LUNAM Univ, Secalim UMR1014, F-44307 Nantes, France. AD - INRA, F-44307 Nantes, France. UR - https://doi.org/10.1016/j.fm.2011.10.013 DO - 10.1016/j.fm.2011.10.013 KW - Seafood KW - Culture-independent method (PCR-TTGE) KW - Sensory analysis KW - Photobacterium phosphoreum KW - Lactococcus piscium N2 - In order to characterise the spoilage related to microbiota of raw salmon, a combination of culture-dependent and -independent methods, including PCR–TTGE, was used to analyse 3 raw salmon batches stored for 3 days at chilled temperature in modified atmosphere packaging (MAP) (50% CO2/50% N2) or under vacuum. Sensory evaluation, microbiological enumeration and chemical analysis were performed after 3, 7 and 10 days of storage. At the onset of spoilage, 65 bacterial isolates were picked from the plates. Thus, 13 different genera or species were identified by phenotypic and molecular tests: Serratia spp., Photobacterium phosphoreum, Yersinia intermedia, Hafnia alvei, Buttiauxella gaviniae, Pseudomonas sp., Carnobacterium maltaromaticum, Carnobacterium divergens, Lactococcus piscium, Lactobacillus fuchuensis, Vagococcus carniphilus, Leuconostoc gasicomitatum and Brochothrix thermosphacta. The PCR–TTGE profiles and band identification enabled a shift of the dominant populations during the storage to be visualised for all the batches, probably due to the temperature change and the packaging. At the beginning of storage, Pseudomonas sp. dominated the raw salmon microbiota while in the following days (7 and 10), P. phosphoreum and L. piscium were identified as the main bacterial groups. This study enhances the knowledge of MAP and vacuum-packed raw salmon spoilage microbiota. Highlights 13 different bacterial taxa were identified by phenotypic and molecular tests. A shift of the dominant microflora during storage was brought to light. The dominant bacterial populations displayed by PCR–TTGE were identified. Y1 - 2012/05 PB - Academic Press Ltd- Elsevier Science Ltd JF - Food Microbiology SN - 0740-0020 VL - 30 IS - 1 SP - 164 EP - 172 ID - 16277 ER -