FN Archimer Export Format
PT J
TI Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms
BT 
AF MARTI, R.
   MIESZKIN, Sophie
   SOLECKI, O.
   POURCHER, A. -M.
   HERVIO-HEATH, Dominique
   GOURMELON, Michele
AS 1:1,2;2:3;3:1,2;4:1,2;5:3;6:3;
FF 1:;2:PDG-RBE-EMP-MIC;3:;4:;5:PDG-RBE-EMP-MIC;6:PDG-RBE-EMP-MIC;
C1 Cemagref, UR GERE, Rennes, France.
   Univ Europeenne Bretagne, Rennes, France.
   IFREMER, EMP, Lab Microbiol, F-29280 Plouzane, France.
C2 CEMAGREF, FRANCE
   UEB, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-RBE-EMP-MIC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
IF 2.337
TC 15
UR https://archimer.ifremer.fr/doc/00053/16470/14534.pdf
LA English
DT Article
DE ;Lactobacillus amylovorus;microbial source tracking;oxygen;persistence;pig-associated Bacteroidales markers;quantitative real-time PCR;river water;temperature
AB Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log reduction (T90). The greatest changes were observed at 20 degrees C under aerobic conditions, leading to a reduction in the diversity of the bacterial populations and in the concentrations of the Pig-1-Bac, Pig-2-Bac and Lactobacillus amylovorus markers with a T90 of 10 5, 8 1 and 17 2 days, respectively. Conclusions: Oxygen and temperature were found to have a combined effect on the persistence of the pig-associated markers in river waters. Significance and Impact of the Study: The persistence profiles of the Pig-1-Bac, Pig-2-Bac and Lact. amylovorus markers in addition to their high specificity and sensitivity support their use as relevant markers to identify pig faecal contamination in river waters.
PY 2011
PD NOV
SO Journal Of Applied Microbiology
SN 1364-5072
PU Wiley-blackwell
VL 111
IS 5
UT 000297020900013
BP 1159
EP 1175
DI 10.1111/j.1365-2672.2011.05131.x
ID 16470
ER

EF