FN Archimer Export Format
PT J
TI Integrating molecular identification of pelagic eggs with geostatistical mapping to improve the delineation of North Sea fish spawning grounds
BT 
AF LELIEVRE, Stephanie
   JEROME, Marc
   MAES, Gregory E.
   VAZ, Sandrine
   CALAIVANY, Sachidhanandam
   VERREZ-BAGNIS, Veronique
AS 1:2;2:1;3:3;4:2;5:1;6:1;
FF 1:PDG-RBE-HMMN-RHBL;2:PDG-RBE-BRM-STBM;3:;4:PDG-RBE-HMMN-RHBL;5:;6:PDG-RBE-BRM-STBM;
C1 IFREMER, Biotechnol & Marine Resources Unit, Nantes, France.
   IFREMER, Fisheries Resources Lab, Boulogne Sur Mer, France.
   Katholieke Univ Leuven, Fish Genet Grp, Lab Biodivers & Evolutionary Genom, Louvain, Belgium.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV KATHOLIEKE LEUVEN, BELGIUM
SI BOULOGNE
   NANTES
SE PDG-RBE-HMMN-RHBL
   PDG-RBE-BRM-STBM
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 2.55
TC 5
UR https://archimer.ifremer.fr/doc/00059/16999/14536.pdf
LA English
DT Article
CR IBTS 2008
BO Thalassa
DE ;Fish eggs;PCR-RFLP;16S rRNA;Geostatistical analyses;Distribution;Spawning grounds
AB Maps of the spawning grounds of commercially important fishes are necessary when assessing the level of connectivity between life stages of fishes and for identifying ecologically valuable marine areas. A first step toward mapping the spawning grounds is a reliable and rapid species identification of pelagic fish eggs to assess the spatio-temporal distribution of spawning aggregations. As many species have similar egg sizes and morphology, the molecular validation of visually identified eggs is often essential for the use of such data in fisheries management. In the present study, we developed a rapid 16S rRNA PCR-restriction fragment length polymorphism (RFLP) assay to distinguish between formalin-fixed fish eggs of dab Limanda limanda, flounder Platichthys flesus and pout Trisopterus spp., which were collected during the 2008 International Bottom Trawl Survey in the Eastern English Channel and southern North Sea. A comparison of the rapid 16S rRNA PCR-RFLP method with initial visual identification revealed 93% of correct identifications for dab, 90% for pout, but only 64% for flounder, representing an overall error rate of 17%. Visual misidentification occurred mainly between dab and flounder and between flounder and pout. Egg abundance and the relative proportions of each species were subsequently analysed geostatistically. Molecular identifications were incorporated to obtain corrected interpolated distribution maps, taking into account the results from molecular identifications as a correction factor. This highlighted the distinct spawning grounds for the 3 studied taxa and facilitated the identification of regions of high conservation value for these species.
PY 2012
SO Marine Ecology-progress Series
SN 0171-8630
PU Inter-research
VL 445
UT 000299384100013
BP 161
EP 172
DI 10.3354/meps09443
ID 16999
ER

EF