FN Archimer Export Format
PT J
TI Pathotyping of Vibrio Isolates by Multiplex PCR Reveals a Risk of Virulent Strain Spreading in New Caledonian Shrimp Farms
BT 
AF LABREUCHE, Yannick
   PALLANDRE, Laurane
   ANSQUER, Dominique
   HERLIN, Jose
   WAPOTRO, Billy
   LE ROUX, Frederique
AS 1:1,2;2:2;3:2;4:2;5:2;6:1;
FF 1:PDG-RBE-LEADNC;2:PDG-RBE-LEADNC-LAC;3:PDG-RBE-LEADNC;4:PDG-RBE-LEADNC;5:;6:PDG-RBE-PFOM;
C1 IFREMER, Equipe Emergente Ifremer UPMC Genom Vibrio, Stn Biol Roscoff FR2424, F-29682 Roscoff, France.
   IFREMER, Dept Lagons Ecosyst & Aquaculture Durable Nouvell, Stn St Vincent, Noumea 98846, New Caledonia.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI SAINT VINCENT
   BREST
SE PDG-RBE-LEADNC
   PDG-RBE-LEADNC-LAC
   PDG-RBE-PFOM
IN WOS Ifremer jusqu'en 2018
IF 3.28
TC 6
UR https://archimer.ifremer.fr/doc/00063/17398/15169.pdf
LA English
DT Article
AB Two recurring syndromes threaten the viability of the shrimp industry in New Caledonia, which represents the second largest export business. The "Syndrome 93" is a cold season disease due to Vibrio penaeicida affecting all shrimp farms, while the "Summer Syndrome" is a geographically restricted vibriosis caused by a virulent lineage of Vibrio nigripulchritudo. Microbiological procedures for diagnosis of these diseases are time-consuming and do not have the ability to discriminate the range of virulence potentials of V. nigripulchritudo. In this study, we developed a multiplex PCR method to simultaneously detect these two bacterial species and allow for pathotype discrimination. The detection limits of this assay, that includes an internal amplification control to eliminate any false-negative results, were determined at 10 pg purified DNA and 200 cfu/ml. After confirming the effectiveness of our method using experimentally infected animals, its accuracy was compared to standard biochemical methods during a field survey using 94 samples collected over 3 years from shrimp farms encountering mortality events. The multiplex PCR showed very high specificity for the detection of V. penaeicida and V. nigripulchritudo (inclusivity and exclusivity 100%) and allowed us to detect the spreading of highly pathogenic isolates of V. nigripulchritudo to a farm adjoining the "Summer Syndrome area." This assay represents a simple, rapid, and cost-effective diagnostic tool for implementing timely risk management decisions but also understanding the seasonal and geographical distribution of these pathogens.
PY 2012
PD JAN
SO Microbial Ecology
SN 0095-3628
PU Springer
VL 63
IS 1
UT 000299084000013
BP 127
EP 138
DI 10.1007/s00248-011-9951-3
ID 17398
ER

EF