FN Archimer Export Format
PT J
TI Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos
BT 
AF AKCHA, Farida
   SPAGNOL, Charlene
   ROUXEL, Julien
AS 1:1;2:1;3:1;
FF 1:PDG-RBE-BE-LBEX;2:PDG-RBE-BE-LBEX;3:PDG-RBE-BE-LBEX;
C1 IFREMER, Lab Ecotoxicol, Dept Biogeochem & Ecotoxicol, F-44311 Nantes 03, France.
C2 IFREMER, FRANCE
SI NANTES
SE PDG-RBE-BE-LBEX
IN WOS Ifremer jusqu'en 2018
IF 3.73
TC 94
UR https://archimer.ifremer.fr/doc/00070/18128/15783.pdf
LA English
DT Article
DE ;Genotoxicity;Embryotoxicity;Oyster;Gametes;Pesticides;Diuron;Glyphosate
AB We investigated the effects of genotoxicant exposure in gametes and embryos to find a possible link between genotoxicity and reproduction/developmental impairment, and explore the impact of chemical genotoxicity on population dynamics. Our study focused on the genotoxic effects of two herbicides on oyster gametes and embryos: glyphosate (both as an active substance and in the Roundup formulation) and diuron. France is Europe's leading consumer of agrochemical substances and as such, contamination of France's coastal waters by pesticides is a major concern. Glyphosate and diuron are among the most frequently detected herbicides in oyster production areas; as oyster is a specie with external reproduction, its gametes and embryos are in direct contact with the surrounding waters and are hence particularly exposed to these potentially dangerous substances. In the course of this study, differences in genotoxic and embryotoxic responses were observed in the various experiments, possibly due to differences in pollutant sensitivity between the tested genitor lots. Glyphosate and Roundup had no effect on oyster development at the concentrations tested, whereas diuron significantly affected embryo-larval development from the lowest tested concentration of 0.05 mu g L-1, i.e. an environmentally realistic concentration. Diuron may therefore have a significant impact on oyster recruitment rates in the natural environment. Our spermiotoxicity study revealed none of the tested herbicides to be cytotoxic for oyster spermatozoa. However, the alkaline comet assay showed diuron to have a significant genotoxic effect on oyster spermatozoa at concentrations of 0.05 mu g L-1 upwards. Conversely, no effects due to diuron exposure were observed on sperm mitochondrial function or acrosomal membrane integrity. Although our initial results showed no negative effect on sperm function, the possible impact on fertilization rate and the consequences of the transmission of damaged DNA for oyster development and physiological performances, requires further investigation. A likely hypothesis to explain the embryotoxic and genotoxic effects of diuron is that it may act via causing oxidative stress. (C) 2011 Elsevier B.V. All rights reserved.
PY 2012
PD JAN
SO Aquatic Toxicology
SN 0166-445X
PU Elsevier Science Bv
VL 106
UT 000300205300013
BP 104
EP 113
DI 10.1016/j.aquatox.2011.10.018
ID 18128
ER

EF