FN Archimer Export Format PT J TI Pharmacological Modulation of Human Mesenchymal Stem Cell Chondrogenesis by a Chemically Oversulfated Polysaccharide of Marine Origin: Potential Application to Cartilage Regenerative Medicine BT AF MERCERON, Christophe PORTRON, Sophie VIGNES-COLOMBEIX, Caroline REDERSTORFF, Emilie MASSON, Martial LESOEUR, Julie SOURICE, Sophie SINQUIN, Corinne COLLIEC-JOUAULT, Sylvia WEISS, Pierre VINATIER, Claire GUICHEUX, Jerome AS 1:2;2:2;3:2;4:2,3;5:2;6:2;7:2;8:3;9:3;10:2;11:2,4;12:1,2; FF 1:;2:;3:;4:;5:;6:;7:;8:PDG-RBE-BRM-BMM;9:PDG-RBE-BRM-BMM;10:;11:;12:; C1 Univ Nantes, Grp STEP Skeletal Tissue Engn & Physiopathol, INSERM, U791,Fac Chirurg Dent,LIOAD,UMRS 791, F-44042 Nantes 1, France. Univ Nantes, UFR Odontol, PRES UNAM, F-44042 Nantes 1, France. IFREMER, Lab Biotechnol & Mol Marines BRM BMM, Nantes, France. GRAFTYS SA, Pole Act Aix en Provence, Aix En Provence, France. C2 UNIV NANTES, FRANCE UNIV NANTES, FRANCE IFREMER, FRANCE GRAFTYS SA, FRANCE SI NANTES SE PDG-DOP-DCN-BRM-BMM PDG-RBE-BRM-BMM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 7.7 TC 51 UR https://archimer.ifremer.fr/doc/00071/18227/17457.pdf LA English DT Article DE ;Cartilage;Adipose-derived mesenchymal stem cells;Polysaccharides;Transforming growth factor-beta AB Mesenchymal stem cells (MSCs) are considered as an attractive source of cells for cartilage engineering due to their availability and capacity for expansion and multipotency. Differentiation of MSC into chondrocytes is crucial to successful cartilage regeneration and can be induced by various biological agents, including polysaccharides that participate in many biological processes through interactions with growth factors. Here, we hypothesize that growth factor-induced differentiation of MSC can be increased by chemically oversulfated marine polysaccharides. To test our hypothesis, human adipose tissue-derived MSCs (hATSCs) were cultured in pellets with transforming growth factor (TGF)-beta 1-supplemented chondrogenic medium containing either the polysaccharide GY785 DR or its oversulfated isoform GY785 DRS. Chondrogenesis was monitored by the measurement of pellet volume, quantification of DNA, collagens, glycosaminoglycans (GAGs), and immunohistological staining. Our data revealed an increase in pellet volume, total collagens, and GAG production with GY785 DRS and chondrogenic medium. The enhanced chondrogenic differentiation of hATSC was further demonstrated by the increased expression of several chondrogenic markers by real-time reverse transcription-polymerase chain reaction. In addition, surface plasmon resonance analyses revealed that TGF-beta 1 bound GY785 DRS with higher affinity compared to GY785 DR. In association with TGF-beta 1, GY785 DRS was found to upregulate the phosphorylation of extracellular signal-regulated kinase 1/2, indicating that oversulfated polysaccharide affects the mitogen activated protein kinase signaling activity. These results demonstrate the upregulation of TGF-beta 1-dependent stem cell chondrogenesis by a chemically oversulfated marine polysaccharide. This polysaccharide of marine origin is easily producible and therefore could be considered a promising additive to drive efficient and reliable MSC chondrogenesis for cartilage tissue engineering. STEM CELLS 2012;30:471-480 PY 2012 PD MAR SO Stem Cells SN 1066-5099 PU Wiley-blackwell VL 30 IS 3 UT 000300611400015 BP 471 EP 480 DI 10.1002/stem.1686 ID 18227 ER EF