FN Archimer Export Format
PT J
TI Effect of starvation on RNA, DNA and protein-content of laboratory-reared larvae and juveniles of Solea-solea
BT 
AF RICHARD, Pierre
   BERGERON, Jean-Pierre
   BOULHIC, Michel
   GALOIS, Robert
   PERSON, Jeanine
AS 1:1;2:2;3:2;4:1,3;5:2;
FF 1:;2:;3:;4:;5:;
C1 CREMA, Centre de Recherche en Ecologie marine et Aquaculture de L'Houmeau, CNRS-IFREMER, B.P. 5, F-17137 L'Houmeau, France
   IFREMER, Centre IFREMER de Brest, B.P. 70, F-29280 Plouzane, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   CNRS, FRANCE
SI NANTES
   BREST
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 1.928
TC 100
UR https://archimer.ifremer.fr/doc/00072/18304/15868.pdf
LA English
DT Article
AB Using analyses of individuals, dry weight, RNA, DNA and protein content were measured on the same samples to assess the nutritional status of laboratory-reared larvae and juveniles of Solea solea (L.). During the early life of S. solea, 2 growth periods could be distinguished by dry weight measurements and biochemical composition analysis. After metamorphosis, dry weight, RNA, DNA and protein content per individual increased more slowly than before metamorphosis. RNA, DNA and protein were strongly positively correlated to dry weight of fed larvae. Starvation led to immediate loss of weight and to decrease in RNA and protein content, while DNA amount per individual remained roughly constant. Percentage of protein relative to dry weight was always lower in starved larvae or juveniles than in fed ones. DNA and RNA percentages decreased after metamorphosis, but remained always higher in starved than in fed fish. The RNA:DNA ratio continuously decreased during starvation and could be used to discriminate starving from feeding larvae after a few days. However, the RNA:DNA ratio of fed larvae showed relatively large fluctuations during larval development, and its decrease during starvation was strongly dependent upon the ontogenetic stage. The increase of DNA relative to dry weight was also dependent upon the developmental stage of starved larvae, but was much more stable in fed larvae and juveniles. Percentage of DNA can be used to define critical levels (3 % before metamorphosis, 1.5 % after it) above which larvae are undergoing starvation. The usefulness of the RNA:DNA ratio or DNA percentage as indices of nutritional condition is discussed.
PY 1991
PD MAY
SO Marine Ecology-progress Series
SN 0171-8630
PU Inter-research
VL 72
IS 1-2
UT A1991FP59800007
BP 69
EP 77
DI 10.3354/meps072069
ID 18304
ER

EF