TY - JOUR T1 - Expression of candidate genes related to metabolism, immunity and cellular stress during massive mortality in the American oyster Crassostrea virginica larvae in relation to biochemical and physiological parameters A1 - Genard,Bertrand A1 - Moraga,Dario A1 - Pernet,Fabrice A1 - David,Elise A1 - Boudry,Pierre A1 - Tremblay,Rejean AD - Univ Quebec, Inst Sci Mer, Rimouski, PQ G5L 3A1, Canada. AD - Univ Bretagne Occidentale, Inst Univ Europeen Mer, Lab Sci Environm Marin LEMAR, Plouzane, France. AD - IFREMER, Lab Environm Ressources Languedoc Roussillon, Sete, France. AD - Univ Reims, Unite Rech Vigne & Vins Champagne Stress & Enviro, UPRES EA2069, Lab Ecol Ecotoxicol, F-51687 Reims 02, France. AD - IFREMER, Lab Physiol Invertebres, F-29280 Plouzane, France. UR - https://doi.org/10.1016/j.gene.2012.02.021 DO - 10.1016/j.gene.2012.02.021 KW - Massive mortality KW - Relative gene expression KW - Metabolism KW - Immunity KW - Cellular stress KW - Larvae N2 - Quantification of mRNA of genes related to metabolism, immunity and cellular stress was examined in relation to a massive mortality event during the culture of American oyster larvae, Crassostrea virginica which was probably, in regard to previous microbiological analysis, induced by vibrio infection. To document molecular changes associated with the mortality event, mRNA levels were compared to biochemical and physiological data, previously described in a companion paper. Among the 18 genes studied, comparatively to the antibiotic control, 10 showed a lower relative gene expression when the massive mortality occurred. Six of them are presumed to be related to metabolism, corroborating the metabolic depression associated with the mortality event suggested by biochemical and physiological analyses. Relationships between the regulation of antioxidant enzyme activities, lipid peroxidation, and the mRNA abundance of genes linked to oxidative stress, cytoprotection, and immune response are also discussed. Finally, we observed an increase in the transcript abundance of two genes involved in apoptosis and cell regulation simultaneously with mortality, suggesting that these processes might be linked. Y1 - 2012/05 PB - Elsevier Science Bv JF - Gene SN - 0378-1119 VL - 499 IS - 1 SP - 70 EP - 75 ID - 18585 ER -