FN Archimer Export Format PT J TI A spatiotemporal study of bacterial community profiles associated with Atlantic bluefin tuna larvae, Thunnus thynnus L., in three Mediterranean hatcheries BT AF GATESOUPE, Joel COVES, Denis ORTEGA, Aurelio PAPANDROULAKIS, Nikos VADSTEIN, Olav DE LA GANDARA, Fernando AS 1:1;2:2;3:3;4:4;5:5;6:3; FF 1:;2:PDG-RBE-BOME-LALR;3:;4:;5:;6:; C1 IFREMER, Ctr Brest, Lab ARN, INRA,UR 1067, F-29280 Plouzane, France. IFREMER, Intrepid, Lab Aquacole Languedoc Roussillon, UMR 110, Palavas Les Flots, France. IEO, Ctr Oceanog Murcia, Puerto De Mazarron, Spain. Hellen Ctr Marine Res, Inst Aquaculture, Iraklion, Greece. Norwegian Univ Sci & Technol NTNU, Dept Biotechnol, Trondheim, Norway. C2 IFREMER, FRANCE IFREMER, FRANCE IEO, SPAIN HELLEN CTR MARINE RES, GREECE UNIV NTNU, NORWAY SI BREST PALAVAS SE PDG-RBE-PFOM-ARN PDG-RBE-BOME-LALR IN WOS Ifremer jusqu'en 2018 copubli-europe IF 1.32 TC 8 UR https://archimer.ifremer.fr/doc/00075/18648/16197.pdf LA English DT Article DE ;larval rearing;bacterial diversity;DGGE;Thunnus thynnus AB During the first 2 years of larval rearing trials with Atlantic bluefin tuna, survival was a challenging issue. As bacterial colonization of the gut has been shown to play a key role for other species, we studied the profiles of the microbiota associated with individual larvae at different stages in three distant hatcheries. The Bacterial Community Profile (BCP) was quantified based on PCR-DGGE analyses of partial amplicons from 16S rDNA. Considerable individual variability in BCP was observed before onset of feeding, and the BCP did not show regular fluctuation during ontogenesis. Microalgae were added to the rearing tanks in two of the three hatcheries, but it was not possible to distinguish the effect of location from the effect of algal addition on BCP. In one hatchery, the larvae were reared either with algal addition or in mesocosm, but due to high individual variability, no significant difference in BCP was detected between the two groups. It was hypothesized that this variability was caused by differences in health, physiological status and developmental stage of the larvae. A practical conclusion from the study is the need to analyse a considerable number of individuals to reflect statistically significant differences between the microbial communities associated with rearing groups. PY 2013 PD SEP SO Aquaculture Research SN 1355-557X PU Wiley-blackwell VL 44 IS 10 UT 000323930400003 BP 1511 EP 1523 DI 10.1111/j.1365-2109.2012.03158.x ID 18648 ER EF