FN Archimer Export Format
PT J
TI Selective elimination of chloroplastidial DNA for metagenomics of bacteria associated with the green alga Caulerpa Taxifolia (bryopsidophyceae)
BT 
AF AIRES, Tania
   MARBA, Nuria
   SERRAO, Ester A.
   DUARTE, Carlos M.
   ARNAUD-HAOND, Sophie
AS 1:1;2:3;3:1;4:3,4;5:1,2;
FF 1:;2:;3:;4:;5:PDG-REM-EEP-LEP;
C1 Univ Algarve, CCMAR Ctr Marine Sci, CIMAR, FCT, P-8005139 Faro, Portugal.
   IFREMER, F-29280 Plouzane, France.
   IMEDEA CSIC UIB Inst Mediterrani Estudis Avancats, Dept Global Change Res, Esporles 07190, Mallorca, Spain.
   Univ Western Australia, UWA Oceans Inst, Crawley 6009, Australia.
C2 UNIV ALGARVE, PORTUGAL
   IFREMER, FRANCE
   IMEDEA, SPAIN
   UNIV WESTERN AUSTRALIA, AUSTRALIA
SI BREST
SE PDG-REM-EEP-LEP
IN WOS Ifremer jusqu'en 2018
   copubli-europe
   copubli-int-hors-europe
IF 2.24
TC 12
UR https://archimer.ifremer.fr/doc/00076/18739/16464.pdf
LA English
DT Article
DE ;16S;chloroplasts;elimination;green algae;metagenomics
AB Molecular analyses of bacteria associated with photosynthetic organisms are often confounded by coamplification of the chloroplastidial 16S rDNA with the targeted bacterial 16S rDNA. This major problem has hampered progress in the characterization of bacterial communities associated to photosynthetic organisms and has limited the full realization of the potential offered by the last generation of metagenomics approaches. A simple and inexpensive method is presented, based on ethanol and bleach treatments prior to extraction, to efficiently discard a great part of chloroplastidial DNA without affecting the characterization of bacterial communities through pyrosequencing. Its effectiveness for the description of bacterial lineages associated to the green alga Caulerpa taxifolia (M. Vahl) C. Agardh was much higher than that of the preexisting enrichment protocols proposed for plants. Furthermore, this new technique requires a very small amount of biological material compared to the other current protocols, making it more realistic for systematic use in ecological and phylogenetic studies and opening promising prospects for metagenomics of green algae, as shown by our data.
PY 2012
PD APR
SO Journal Of Phycology
SN 0022-3646
PU Wiley-blackwell
VL 48
IS 2
UT 000302467900025
BP 483
EP 490
DI 10.1111/j.1529-8817.2012.01124.x
ID 18739
ER

EF