FN Archimer Export Format
PT J
TI Quantitative analysis of azaspiracids in Azadinium spinosum cultures
OT Analyse quantitative d'azaspiracides dans des cultures d'Azadinium spinosum
BT 
AF JAUFFRAIS, Thierry
   HERRENKNECHT, Christine
   SECHET, Veronique
   SIBAT, Manoella
   TILLMANN, Urban
   KROCK, Bernd
   KILCOYNE, Jane
   MILES, Christopher O.
   MCCARRON, Pearse
   AMZIL, Zouher
   HESS, Philipp
AS 1:1;2:2;3:1;4:1;5:3;6:3;7:4;8:5;9:6;10:1;11:1;
FF 1:PDG-RBE-EMP-PHYC;2:;3:PDG-RBE-EMP-PHYC;4:PDG-RBE-EMP-PHYC;5:;6:;7:;8:;9:;10:PDG-RBE-EMP-PHYC;11:PDG-ODE-LITTORAL-PHYC;
C1 IFREMER, Lab EMP PHYC, F-44311 Nantes, France.
   Nantes Atlantique Univ, MMS EA2160, F-44035 Nantes, France.
   Alfred Wegener Inst, D-27570 Bremerhaven, Germany.
   Oranmore Co, Inst Marine, Rinville, Galway, Ireland.
   Norwegian Vet Inst, N-0106 Oslo, Norway.
   Natl Res Council Canada, Halifax, NS B3H 3Z1, Canada.
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
   INST A WEGENER, GERMANY
   MARINE INST GALWAY, IRELAND
   NORWEGIAN VET INST, NORWAY
   NATL RES COUNCIL CANADA, CANADA
SI NANTES
SE PDG-RBE-EMP-PHYC
   PDG-ODE-LITTORAL-PHYC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
IF 3.66
TC 27
UR https://archimer.ifremer.fr/doc/00077/18812/16463.pdf
LA English
DT Article
DE ;Extraction procedure;Extraction artefact;Matrix effects;LC-MS/MS;Azaspiracid methyl ester;Dinoflagellate;Liquid chromatography-mass spectrometry
AB Azaspiracids (AZAs) are secondary metabolites of Azadinium spinosum, that have been shown to cause diarrhetic shellfish poisoning when accumulated in bivalve molluscs. We describe here an analytical procedure for the determination of AZAs in cultures of A. spinosum with a focus on the potential formation of AZA methyl-esters as artefacts in the extraction and sample pre-treatment. A. spinosum cells were collected from bioreactor cultures, using centrifugation or filtration. Different extraction procedures were evaluated for formation of methyl-ester artefacts, yield, and matrix effects. Filtration of cultures using glass-fibre filters led to increased formation of methyl-esters. Hence centrifugation is recommended for recovery of cells. The type of extraction solvent (MeOH, Acetone, ACN) did not significantly affect the yield of AZAs so long as the organic content was 80% or higher. However, the use of MeOH as extraction solvent led to increased formation of methyl-ester artefacts. AZA1 recovery over two successive extractions was 100% at 95% confidence level for acetone and MeOH. In standard addition experiments no significant matrix effects were observed in extracts of A. spinosum or A. obesum up to a sample intake of 4.5*109 µm3. Moreover, experiments carried out to clarify the formation and structure of methylated AZA analogues, led to the description of two new AZAs and the correction of the chemical structures of AZA29-32.
PY 2012
PD MAY
SO Analytical And Bioanalytical Chemistry
SN 1618-2642
PU Springer Heidelberg
VL 403
IS 3
UT 000303409600023
BP 833
EP 846
DI 10.1007/s00216-012-5849-2
ID 18812
ER

EF