Seasonal variability in trypsin and alpha-amylase activities caused by the molting cycle and feeding habits of juvenile pink shrimp Farfantepenaeus duorarum (Burkenroad, 1939)

Type Article
Date 2012-02
Language English
Author(s) Aragon-Axomulco Heron1, Chiappa-Carrara Xavier2, Soto Luis3, Cuzon GerardORCID4, Arena Leticia2, Maldonado Carlos1, Cardenas Rene5, Gaxiola Gabriela2
Affiliation(s) 1 : Univ Nacl Autonoma Mexico, Fac Ciencias, Unidad Multidisciplinaria Docencia & Invest Sisal, Hunucma 97355, Yucatan, Mexico.
2 : Univ Nacl Autonoma Mexico, Inst Ciencias Mar & Limnol, Hunucma 97355, Yucatan, Mexico.
3 : Universidad Nacional Autónoma de México,, nstituto de Ciencias del Mar y Limnología, Puerto de Abrigo S/N, Sisal, Hunucmá, Yucatán 97355, México
4 : IFREMER, Ctr Pacifique, Taravao 98719, Tahiti, Fr Polynesia.
5 : Universidad Nacional Autónoma de México, Facultad de Ciencias, Puerto de Abrigo S/N, Sisal, Hunucmá, Yucatán 97355, México
Source Journal Of Crustacean Biology (0278-0372) (Crustacean Society), 2012-02 , Vol. 32 , N. 1 , P. 89-99
DOI 10.1163/193724011X615361
WOS© Times Cited 11
Keyword(s) coastal lagoons, digestive enzymes, Farfantepenaeus duorarum, feeding habits, pink shrimp
Abstract In view of the relationship between shifts in diet composition and the activity of digestive enzymes in penaeid shrimp, the present study focused on the analysis of digestive trypsin and a-amylase activities of wild Farfantepenaeus. duorarum (Burkenroad, 1939) juveniles and their changes in phenotypic expression, during the molt cycle as endogenous factor and their changes due to different feeding regimes (exogenous factor) in relation with delta C-13 and delta N-15 isotopic signature as an index of food assimilation induced by the seasonal availability of food items in the nursery area. Wild juveniles of F. duorarum were captured from April 2007 to February 2008, in the Celestun coastal lagoon, Yucatan, Mexico. Samplings were carried out considering all quarters of the lunar cycle and in each of the recognized seasons for this region: dry, rainy, and the Nortes (North Wind). Copepods and amphipods were the main source of food for juveniles of E duorarum. Values of delta C-13 in the muscular tissue were near -20 parts per thousand hence the feeding regime of F. duorarum in the lagoon was composed by material of marine origin. Isotopic signature differences were found between the three annual seasons. It is an opportunist generalist organism that is located in the 4(th) trophic level. The digestive enzymatic activities of both trypsin and alpha-amylase in fresh hepatopancreas tissue showed an interaction between season and molt stages (p < 0.05). Activity of the trypsin was highest during the Nortes at molt stage C (140 mU mg(-1) HP) and activity of a-amylase was higher in the Nortes at stage B-2 (674 mU mg(-1) HP). The amylase/trypsin ratio also showed significant interaction between season and molt stages (p < 0.05), with higher values in premolt stages during the rainy and Nortes seasons. Isoforms of these digestive enzymes differed in expression according to the molt stage and also to the season with expression generally being greater at stage C.
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Aragon-Axomulco Heron, Chiappa-Carrara Xavier, Soto Luis, Cuzon Gerard, Arena Leticia, Maldonado Carlos, Cardenas Rene, Gaxiola Gabriela (2012). Seasonal variability in trypsin and alpha-amylase activities caused by the molting cycle and feeding habits of juvenile pink shrimp Farfantepenaeus duorarum (Burkenroad, 1939). Journal Of Crustacean Biology, 32(1), 89-99. Publisher's official version : https://doi.org/10.1163/193724011X615361 , Open Access version : https://archimer.ifremer.fr/doc/00078/18880/