Fitness of early life stages in F1 interspecific hybrids between Dicentrarchus labrax and D. punctatus

Type Article
Date 2012-01
Language English
Author(s) Ky Chin-Long1, Vergnet Alain2, Molinari Nicolas3, Fauvel Christian2, Bonhomme Francois4
Affiliation(s) 1 : IFREMER, Ctr Pacifique, Dep Ressources Biol & Environm, UR Ressources Marines Polynesie Francaise, Taravao 98719, Tahiti, Fr Polynesia.
2 : IFREMER, Univ Montpellier 2, CC093, UM2 UM1 CNRS IRD IFREMER, UMR ECOSYM 5119, F-34095 Montpellier 5, France.
3 : Univ Montpellier 1, CHRU Montpellier, Serv DIM, Montpellier SupAgro,INRA,MISTEA UMR729, F-34060 Montpellier, France.
4 : Univ Montpellier 2, CC 63, UMR 5554, Inst Sci Evolut,Dept Biol Integrat, F-34060 Montpellier 5, France.
Source Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2012-01 , Vol. 25 , N. 1 , P. 67-75
DOI 10.1051/alr/2012006
WOS© Times Cited 7
Keyword(s) Sea bass, Hybrid progeny, Offspring quality, Fitness, Dicentrarchus labrax, Dicentrarchus punctatus
Abstract Inter- and intraspecific crossbreeding experiments were conducted to evaluate the aquaculture potential of hybrids in the genus Dicentrarchus, focusing on fertilisation and hatching success. The experimental design consisted of 24 controlled crosses in which individual and pooled fertilisations were made between wild D. labrax individuals (8 dams and 5 sires originating from West Mediterranean and Atlantic populations) and wild D. punctatus (6 sires). Three experiments were successively performed: (1) dams from the Mediterranean population, individually considered to have good egg quality, (2) dams from Mediterranean population, individually considered to have poor egg quality and (3) crossbreeding using pooled eggs from the Atlantic population. In each case, batches of these eggs were fertilized by sperm from males of the two species. Sperm cell quality (concentration and motility) was verified before experimentation in all cases and equal numbers of sperm cells from each male were used to individually (without inter-sire competition) fertilise egg batches. Through the repeated artificial crosses between female common sea bass Dicentrarchus labrax (Linnaeus 1758) and male spotted sea bass D. punctatus (Block 1792), these experiments showed that no post-zygotic reproductive barriers exist to interspecific hybridisation between these two species when using D. labrax as dams and D. punctatus as sires. Phenotypically, the F1 hybrids were easily recognisable: they inherited the characteristic black spots of D. punctatus. Furthermore, embryo survival was significantly higher in interspecific crosses compared with intraspecific controls, showing increased fitness for this trait (increased performance at early life stage). Then, the experimental breeding design validates the observation that West Mediterranean and Atlantic common sea bass populations are different. Indeed, the inter-population crosses (between West Mediterranean dams and Atlantic sires) also revealed increased fitness at early life stages in comparison with the progeny of intra-Atlantic population crosses. However, these individuals were still smaller than their interspecific counterparts. The ability to produce viable F1 hybrids will have significant implications for the practical improvement of sea bass aquaculture.
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