Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas

Type Article
Date 2012-04
Language English
Author(s) Bigot Laetitia1, 2, Zatylny-Gaudin Celine1, 2, Rodet Franck3, Bernay Benoit1, Boudry PierreORCID4, Favrel Pascal1, 2
Affiliation(s) 1 : Univ Caen Basse Normandie, IBFA, Biol Mollusques Marins & Ecosyst Associes BioMEA, F-14032 Caen, France.
2 : CNRS INEE FRE 3484, BioMEA, Paris, France.
3 : Univ Lille 1, Lab Spectrometrie Masse Biol Fondamentale & Appl, EA4550, IFR 147, F-59650 Villeneuve Dascq, France.
4 : IFREMER, Unite Physiol Fonct Organismes Marins, F-29280 Plouzane, France.
Source Peptides (0196-9781) (Elsevier Science Inc), 2012-04 , Vol. 34 , N. 2 , P. 303-310
DOI 10.1016/j.peptides.2012.01.017
WOS© Times Cited 36
Keyword(s) Mollusk, Bivalve, Oyster, Neuroendocrinology, Reproduction, GnRH
Abstract Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the precursor protein were exclusively expressed in the visceral ganglia. Significant differences in transcriptional activity of Cg-GnRH encoding gene were recorded in the ganglia along the reproductive cycle and according to trophic conditions with a higher level in fed animals compared to starved animals. This suggests the involvement of Cg-GnRHs as synchronizers of nutritional status with energy requirements during reproduction in oyster. Evidence for a role of Cg-GnRHs as neuroregulators and as neuroendocrine factors in bivalve is discussed. (C) 2012 Elsevier Inc. All rights reserved.
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