FN Archimer Export Format
PT J
TI Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas
BT 
AF BIGOT, Laetitia
   ZATYLNY-GAUDIN, Celine
   RODET, Franck
   BERNAY, Benoit
   BOUDRY, Pierre
   FAVREL, Pascal
AS 1:1,2;2:1,2;3:3;4:1;5:4;6:1,2;
FF 1:;2:;3:;4:;5:PDG-RBE-PFOM-PI;6:;
C1 Univ Caen Basse Normandie, IBFA, Biol Mollusques Marins & Ecosyst Associes BioMEA, F-14032 Caen, France.
   CNRS INEE FRE 3484, BioMEA, Paris, France.
   Univ Lille 1, Lab Spectrometrie Masse Biol Fondamentale & Appl, EA4550, IFR 147, F-59650 Villeneuve Dascq, France.
   IFREMER, Unite Physiol Fonct Organismes Marins, F-29280 Plouzane, France.
C2 UNIV CAEN, FRANCE
   CNRS, FRANCE
   UNIV LILLE, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-RBE-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.52
TC 42
UR https://archimer.ifremer.fr/doc/00079/19003/17477.pdf
LA English
DT Article
DE ;Mollusk;Bivalve;Oyster;Neuroendocrinology;Reproduction;GnRH
AB Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the precursor protein were exclusively expressed in the visceral ganglia. Significant differences in transcriptional activity of Cg-GnRH encoding gene were recorded in the ganglia along the reproductive cycle and according to trophic conditions with a higher level in fed animals compared to starved animals. This suggests the involvement of Cg-GnRHs as synchronizers of nutritional status with energy requirements during reproduction in oyster. Evidence for a role of Cg-GnRHs as neuroregulators and as neuroendocrine factors in bivalve is discussed. (C) 2012 Elsevier Inc. All rights reserved.
PY 2012
PD APR
SO Peptides
SN 0196-9781
PU Elsevier Science Inc
VL 34
IS 2
UT 000303181600005
BP 303
EP 310
DI 10.1016/j.peptides.2012.01.017
ID 19003
ER

EF