Analysis of Clinical Ostreid Herpesvirus 1 (Malacoherpesviridae) Specimens by Sequencing Amplified Fragments from Three Virus Genome Areas

Type Article
Date 2012-05
Language English
Author(s) Renault Tristan1, Moreau Pierrick1, Faury Nicole1, Pepin Jean-Francois1, Segarra Amelie1, Webb Stephen2
Affiliation(s) 1 : IFREMER, Lab Genet & Pathol, Ronce Bains, Ronce Les Bains, La Tremblade, France.
2 : Cawthron Inst, Nelson, New Zealand.
Source Journal Of Virology (0022-538X) (Amer Soc Microbiology), 2012-05 , Vol. 86 , N. 10 , P. 5942-5947
DOI 10.1128/JVI.06534-11
WOS© Times Cited 95
Abstract Although there are a number of ostreid herpesvirus 1 (OsHV-1) variants, it is expected that the true diversity of this virus will be known only after the analysis of significantly more data. To this end, we analyzed 72 OsHV-1 "specimens" collected mainly in France over an 18-year period, from 1993 to 2010. Additional samples were also collected in Ireland, the United States, China, Japan, and New Zealand. Three virus genome regions (open reading frame 4 [ORF4], ORF35, -36, -37, and -38, and ORF42 and -43) were selected for PCR analysis and sequencing. Although ORF4 appeared to be the most polymorphic genome area, distinguishing several genogroups, ORF35, -36, -37, and -38 and ORF42 and -43 also showed variations useful in grouping subpopulations of this virus.
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Renault Tristan, Moreau Pierrick, Faury Nicole, Pepin Jean-Francois, Segarra Amelie, Webb Stephen (2012). Analysis of Clinical Ostreid Herpesvirus 1 (Malacoherpesviridae) Specimens by Sequencing Amplified Fragments from Three Virus Genome Areas. Journal Of Virology, 86(10), 5942-5947. Publisher's official version : https://doi.org/10.1128/JVI.06534-11 , Open Access version : https://archimer.ifremer.fr/doc/00083/19445/