TY - JOUR T1 - Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure A1 - Bougaran,Gael A1 - Rouxel,Catherine A1 - Dubois,Nolwenn A1 - Kaas,Raymond A1 - Grouas,Sophie A1 - Lukomska,Ewa A1 - Le Coz,Jean-Rene A1 - Cadoret,Jean-Paul AD - IFREMER, PBA, F-44311 Nantes 03, France. AD - IFREMER, LPI, UMR100, PE2M, F-29280 Plouzane, France. UR - https://doi.org/10.1002/bit.24560 DO - 10.1002/bit.24560 KW - strain selection KW - Isochrysis KW - neutral lipid KW - ultra violet KW - flux cytometry KW - biofuel N2 - Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...) Y1 - 2012/11 PB - Wiley-blackwell JF - Biotechnology And Bioengineering SN - 0006-3592 VL - 109 IS - 11 SP - 2737 EP - 2745 ID - 19643 ER -