FN Archimer Export Format PT J TI Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure BT AF BOUGARAN, Gael ROUXEL, Catherine DUBOIS, Nolwenn KAAS, Raymond GROUAS, Sophie LUKOMSKA, Ewa LE COZ, Jean-Rene CADORET, Jean-Paul AS 1:1;2:1;3:1;4:1;5:1;6:1;7:2;8:1; FF 1:PDG-RBE-BRM-PBA;2:PDG-RBE-BRM-PBA;3:PDG-DOP-DCN-BRM-PBA;4:PDG-RBE-BRM-PBA;5:PDG-DOP-DCB-BM-PBA;6:PDG-RBE-BRM-PBA;7:PDG-RBE-PFOM-PI;8:PDG-RBE-BRM-PBA; C1 IFREMER, PBA, F-44311 Nantes 03, France. IFREMER, LPI, UMR100, PE2M, F-29280 Plouzane, France. C2 IFREMER, FRANCE IFREMER, FRANCE SI NANTES BREST SE PDG-RBE-BRM-PBA PDG-DOP-DCN-BRM-PBA PDG-DOP-DCB-BM-PBA PDG-RBE-PFOM-PI IN WOS Ifremer jusqu'en 2018 IF 3.65 TC 55 UR https://archimer.ifremer.fr/doc/00085/19643/17418.pdf https://archimer.ifremer.fr/doc/00085/19643/98092.pdf LA English DT Article DE ;strain selection;Isochrysis;neutral lipid;ultra violet;flux cytometry;biofuel AB Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...) PY 2012 PD NOV SO Biotechnology And Bioengineering SN 0006-3592 PU Wiley-blackwell VL 109 IS 11 UT 000309185600005 BP 2737 EP 2745 DI 10.1002/bit.24560 ID 19643 ER EF