TY - JOUR T1 - In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas A1 - Huvet,Arnaud A1 - Fleury,Elodie A1 - Corporeau,Charlotte A1 - Quillien,Virgile A1 - Daniel,Jean-Yves A1 - Riviere,Guillaume A1 - Boudry,Pierre A1 - Fabioux,Caroline AD - IFREMER, UMR Physiol & Ecophysiol Mollusques Marins, F-292800 Plouzane, France. AD - IFREMER, Stn Trinite Mer, LER MPL, F-56470 La Trinite Sur Mer, France. AD - Univ Caen Basse Normandie, UMR Physiol & Ecophysiol Mollusques Marins, F-14032 Caen, France. AD - Univ Bretagne Occidentale, Inst Univ Europeen Mer, LEMAR, UMR CNRS UBO IRD 6539, F-29280 Plouzane, France. UR - https://doi.org/10.1007/s10126-011-9421-4 DO - 10.1007/s10126-011-9421-4 KW - Marine bivalve KW - Crassostrea gigas KW - Reproduction KW - RNA interference KW - Transforming growth factor-beta N2 - We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated into the gonad, likely as part of the active resorption of degenerating germ cells. Conversely, males showed a normal phenotype at the cellular level, with spermatids and spermatozoids observed in the gonads of control and injected males. As a result, og-TGF beta appears to play an essential role in C. gigas germ cell development by functioning as an activator of germ cell proliferation in both male and female oysters and vitellogenesis in females. Y1 - 2012/08 PB - Springer JF - Marine Biotechnology SN - 1436-2228 VL - 14 IS - 4 SP - 402 EP - 410 ID - 19794 ER -