FN Archimer Export Format PT J TI In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas BT AF HUVET, Arnaud FLEURY, Elodie CORPOREAU, Charlotte QUILLIEN, Virgile DANIEL, Jean-Yves RIVIERE, Guillaume BOUDRY, Pierre FABIOUX, Caroline AS 1:1;2:2;3:1;4:1;5:1;6:3;7:1;8:4; FF 1:PDG-RBE-PFOM-PI;2:PDG-ODE-LER-LERMPL;3:PDG-RBE-PFOM-PI;4:PDG-RBE-PFOM-PI;5:PDG-RBE-PFOM-PI;6:;7:PDG-RBE-PFOM-PI;8:; C1 IFREMER, UMR Physiol & Ecophysiol Mollusques Marins, F-292800 Plouzane, France. IFREMER, Stn Trinite Mer, LER MPL, F-56470 La Trinite Sur Mer, France. Univ Caen Basse Normandie, UMR Physiol & Ecophysiol Mollusques Marins, F-14032 Caen, France. Univ Bretagne Occidentale, Inst Univ Europeen Mer, LEMAR, UMR CNRS UBO IRD 6539, F-29280 Plouzane, France. C2 IFREMER, FRANCE IFREMER, FRANCE UNIV CAEN, FRANCE UBO, FRANCE SI BREST LA TRINITE SE PDG-RBE-PFOM-PI PDG-ODE-LER-LERMPL IUEM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.74 TC 24 UR https://archimer.ifremer.fr/doc/00087/19794/17486.pdf LA English DT Article DE ;Marine bivalve;Crassostrea gigas;Reproduction;RNA interference;Transforming growth factor-beta AB We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated into the gonad, likely as part of the active resorption of degenerating germ cells. Conversely, males showed a normal phenotype at the cellular level, with spermatids and spermatozoids observed in the gonads of control and injected males. As a result, og-TGF beta appears to play an essential role in C. gigas germ cell development by functioning as an activator of germ cell proliferation in both male and female oysters and vitellogenesis in females. PY 2012 PD AUG SO Marine Biotechnology SN 1436-2228 PU Springer VL 14 IS 4 UT 000305222400003 BP 402 EP 410 DI 10.1007/s10126-011-9421-4 ID 19794 ER EF