Gametogenesis in the Pacific Oyster Crassostrea gigas: A Microarrays-Based Analysis Identifies Sex and Stage Specific Genes

Type Article
Date 2012-05
Language English
Author(s) Dheilly Nolwenn1, 2, 3, Lelong Christophe1, 2, Huvet ArnaudORCID3, Kellner Kristell1, 2, Dubos Marie-Pierre1, 2, Riviere Guillaume1, 2, Boudry PierreORCID3, Favrel Pascal1, 2
Affiliation(s) 1 : Univ Caen Basse Normandie, IBFA, SFR ICORE, Biol Organismes Marins & Ecosyst Associes BioMEA, Caen, France.
2 : CNRS, INEE, BioMEA, Caen, France.
3 : IFREMER, Lab Sci Environm Marin, Ctr Bretagne, Plouzane, France.
Source Plos One (1932-6203) (Public Library Science), 2012-05 , Vol. 7 , N. 5
DOI 10.1371/journal.pone.0036353
WOS© Times Cited 37
Abstract Background: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and little more about the molecular pathways regulating reproduction. We have recently developed and validated a microarray containing 31,918 oligomers (Dheilly et al., 2011) representing the oyster transcriptome. The application of this microarray to the study of mollusk gametogenesis should provide a better understanding of the key factors involved in sex differentiation and the regulation of oyster reproduction. Methodology/Principal Findings: Gene expression was studied in gonads of oysters cultured over a yearly reproductive cycle. Principal component analysis and hierarchical clustering showed a significant divergence in gene expression patterns of males and females coinciding with the start of gonial mitosis. ANOVA analysis of the data revealed 2,482 genes differentially expressed during the course of males and/or females gametogenesis. The expression of 434 genes could be localized in either germ cells or somatic cells of the gonad by comparing the transcriptome of female gonads to the transcriptome of stripped oocytes and somatic tissues. Analysis of the annotated genes revealed conserved molecular mechanisms between mollusks and mammals: genes involved in chromatin condensation, DNA replication and repair, mitosis and meiosis regulation, transcription, translation and apoptosis were expressed in both male and female gonads. Most interestingly, early expressed male-specific genes included bindin and a dpy-30 homolog and female-specific genes included foxL2, nanos homolog 3, a pancreatic lipase related protein, cd63 and vitellogenin. Further functional analyses are now required in order to investigate their role in sex differentiation in oysters. Conclusions/Significance: This study allowed us to identify potential markers of early sex differentiation in the oyster C. gigas, an alternative hermaphrodite mollusk. We also provided new highly valuable information on genes specifically expressed by mature spermatozoids and mature oocytes.
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Dheilly Nolwenn, Lelong Christophe, Huvet Arnaud, Kellner Kristell, Dubos Marie-Pierre, Riviere Guillaume, Boudry Pierre, Favrel Pascal (2012). Gametogenesis in the Pacific Oyster Crassostrea gigas: A Microarrays-Based Analysis Identifies Sex and Stage Specific Genes. Plos One, 7(5). Publisher's official version : https://doi.org/10.1371/journal.pone.0036353 , Open Access version : https://archimer.ifremer.fr/doc/00087/19862/