FN Archimer Export Format
PT J
TI Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas
BT 
AF HUVET, Arnaud
   JEFFROY, Fanny
   DANIEL, Jean-Yves
   QUERE, Claudie
   LE SOUCHU, Pierrick
   VAN WORMHOUDT, A.
   BOUDRY, Pierre
   MOAL, Jeanne
   SAMAIN, Jean-Francois
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:1;8:1;9:1;
FF 1:PDG-RBE-PFOM-PI;2:PDG-DOP-DCB-PFOM-PI;3:PDG-RBE-PFOM-PI;4:PDG-RBE-PFOM-PI;5:PDG-RBE-PFOM-PI;6:;7:PDG-RBE-PFOM-PI;8:PDG-RBE-PFOM-PI;9:PDG-DOP-DCB-PFOM-PI;
C1 IFREMER, UMR CNRS 6539, Lab Sci Environm Marin, F-29280 Plouzane, France.
   Museum Natl Hist Nat, Stn Biol Marine, F-29900 Concarneau, France.
C2 IFREMER, FRANCE
   MNHN, FRANCE
SI BREST
   ARGENTON
SE PDG-RBE-PFOM-PI
   PDG-DOP-DCB-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 2.07
TC 11
UR https://archimer.ifremer.fr/doc/00087/19869/17521.pdf
LA English
DT Article
DE ;alpha-amylase;Crassostrea gigas;digestive enzyme;Michaelis-Menten constant;regulation
AB In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to food availability, whereas the functional role of AMYB was still unknown. To improve knowledge about the regulation of amylase expression in C. gigas and the respective roles of the two genes, we made an assay of amylase expression at mRNA and enzymatic levels in the digestive gland of oysters that had received dietary supplements of starch. After 18 days, a significant increase of translatable mRNA for AMYB was observed, with a correlated increase in Michaelis-Menten constant Km values and a decrease in total amylase activity. This modulation is the first evidence of observable functioning of AMYB in digestive processes. Amylase B is suggested to display a higher Km than amylase A, offering a means of adapting to high substrate concentrations. The highest starch supplement level (10 mg L− 1) induced alteration in oyster physiology. The 1 mg L− 1 treatment should be tested as a practical food supplement that could lead to growth benefits for oysters.
PY 2012
PD SEP
SO Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology
SN 1096-4959
PU Elsevier Science Inc
VL 163
IS 1
UT 000307030000010
BP 96
EP 100
DI 10.1016/j.cbpb.2012.05.003
ID 19869
ER

EF