FN Archimer Export Format
PT J
TI Production and Isolation of Azaspiracid-1 and -2 from Azadinium spinosum Culture in Pilot Scale Photobioreactors
BT 
AF JAUFFRAIS, Thierry
   KILCOYNE, Jane
   SECHET, Veronique
   HERRENKNECHT, Christine
   TRUQUET, Philippe
   HERVE, Fabienne
   BERARD, Jean-Baptiste
   NULTY, Ciara
   TAYLOR, Sarah
   TILLMANN, Urban
   MILES, Christopher O.
   HESS, Philipp
AS 1:1;2:6;3:1;4:2;5:1;6:1;7:3;8:6;9:1;10:4;11:5;12:1;
FF 1:PDG-RBE-EMP-PHYC;2:;3:PDG-RBE-EMP-PHYC;4:;5:PDG-RBE-EMP-PHYC;6:PDG-RBE-EMP-PHYC;7:PDG-RBE-BRM-PBA;8:;9:;10:;11:;12:PDG-ODE-LITTORAL-PHYC;
C1 IFREMER, EMP PHYC Lab, F-44311 Nantes, France.
   Nantes Atlantic Univ, MMS EA2160, F-44035 Nantes, France.
   IFREMER, BRM PBA Lab, F-44311 Nantes, France.
   Alfred Wegener Inst, D-27570 Bremerhaven, Germany.
   Norwegian Vet Inst, N-0106 Oslo, Norway.
   Inst Marine, Oranmore, Co Galway, Ireland.
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
   IFREMER, FRANCE
   INST A WEGENER, GERMANY
   NORWEGIAN VET INST, NORWAY
   MARINE INST GALWAY, IRELAND
SI NANTES
SE PDG-RBE-EMP-PHYC
   PDG-RBE-BRM-PBA
   PDG-ODE-LITTORAL-PHYC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 3.98
TC 23
UR https://archimer.ifremer.fr/doc/00088/19875/17526.pdf
   https://archimer.ifremer.fr/doc/00088/19875/17527.pdf
LA English
DT Article
DE ;solid phase extraction;photobioreactor;chemostat;dinoflagellate;micro-algae;LC-MS/MS;tangential flow filtration;azaspiracid;HP-20
AB Azaspiracid (AZA) poisoning has been reported following consumption of contaminated shellfish, and is of human health concern. Hence, it is important to have sustainable amounts of the causative toxins available for toxicological studies and for instrument calibration in monitoring programs, without having to rely on natural toxin events. Continuous pilot scale culturing was carried out to evaluate the feasibility of AZA production using Azadinium spinosum cultures. Algae were harvested using tangential flow filtration or continuous centrifugation. AZAs were extracted using solid phase extraction (SPE) procedures, and subsequently purified. When coupling two stirred photobioreactors in series, cell concentrations reached 190,000 and 210,000 cell·mL−1 at steady state in bioreactors 1 and 2, respectively. The AZA cell quota decreased as the dilution rate increased from 0.15 to 0.3 day−1, with optimum toxin production at 0.25 day−1. After optimization, SPE procedures allowed for the recovery of 79 ± 9% of AZAs. The preparative isolation procedure previously developed for shellfish was optimized for algal extracts, such that only four steps were necessary to obtain purified AZA1 and -2. A purification efficiency of more than 70% was achieved, and isolation from 1200 L of culture yielded 9.3 mg of AZA1 and 2.2 mg of AZA2 of >95% purity. This work demonstrated the feasibility of sustainably producing AZA1 and -2 from A. spinosum cultures.
PY 2012
PD JUL
SO Marine Drugs
SN 1660-3397
PU Mdpi Ag
VL 10
IS 6
UT 000305799800013
BP 1360
EP 1382
DI 10.3390/md10061360
ID 19875
ER

EF