FN Archimer Export Format PT J TI Evaluation of the partial renewal of in situ phytoplankton microcosms and application to the impact assessment of bentazon and dimethenamid BT AF DE LA BROISE, Denis STACHOWSKI-HABERKORN, Sabine AS 1:1;2:2; FF 1:;2:PDG-RBE-BE-LBEX; C1 Univ Bretagne Occidentale, LEMAR, IUEM, UEB, F-29280 Plouzane, France. IFREMER Lab Ecotoxicol, F-44311 Nantes, France. C2 UBO, FRANCE IFREMER, FRANCE SI NANTES SE PDG-RBE-BE-LBEX IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.53 TC 2 UR https://archimer.ifremer.fr/doc/00088/19889/17548.pdf LA English DT Article DE ;Phytoplankton;Microcosm;Herbicides;Dimethenamid;Bentazon AB Microcosms, each consisting of 2 L natural surface seawater maintained in 2.3-L glass bottles, were immersed at a depth of 6 metres. The renewal of ten percent of microcosm volumes was carried out every other day. Phytoplankton-containing seawater was used for renewal (previously filtered through 25-, 50- or 200-μm cut-off). Phytoplankton community pigment analysis (by HPLC) and flow cytometry analysis were performed. After 13 days, data exhibited phytoplankton characteristics in microcosms in the same range as that of the natural surrounding sea water over the same period. Furthermore, in these microcosms, a negative correlation was observed between the filtration cut-off used for renewal water, and the eukaryote cell count. Herbicides were tested as commercial mixtures at 1, 10 and 100 μg.L-1 active principal. Both Frontier® (dimethenamid) and Basamais® (bentazon) induced significant modifications of the phytoplankton populations at every concentration tested. Such results suggest a possible disturbance in polluted coastal areas. PY 2012 PD NOV SO Marine Pollution Bulletin SN 0025-326X PU Pergamon-elsevier Science Ltd VL 64 IS 11 UT 000312418100037 BP 2480 EP 2488 DI 10.1016/j.marpolbul.2012.07.039 ID 19889 ER EF