FN Archimer Export Format
PT J
TI Setting tools for the early assessment of the quality of thawed Pacific oyster (Crassostrea gigas) D-larvae
BT 
AF SUQUET, Marc
   LE MERCIER, Alain
   RIMOND, Flore
   MINGANT, Christian
   HAFFRAY, Pierrick
   LABBE, C.
AS 1:1;2:2;3:1,2;4:1;5:3;6:4;
FF 1:PDG-RBE-PFOM-PI;2:;3:PDG-RBE-HMMN-RHPEB;4:PDG-RBE-PFOM-PI;5:;6:;
C1 IFREMER, PFOM Dept, Stn Expt Argenton, UMR 6539, Argenton, France.
   IUEM, Lemar, UMR 6539, Plouzane, France.
   SYSAAF, Scribe, Rennes, France.
   INRA, UR LPGP 1037, F-35042 Rennes, France.
C2 IFREMER, FRANCE
   UBO, FRANCE
   SYSAAF, FRANCE
   INRA, FRANCE
SI ARGENTON
   PORT-EN-BESSIN
SE PDG-RBE-PFOM-PI
   PDG-RBE-HMMN-RHPEB
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
IF 2.08
TC 18
UR https://archimer.ifremer.fr/doc/00088/19934/17761.pdf
LA English
DT Article
DE ;ASMA;CASA;Crassostrea gigas;Cryopreservation;D-larvae
AB Parameters used to assess the survival of larvae after cryopreservation generally misestimate the damages that prevent larval development. The objectives of the present study were to 1) define the reliability of the survival rate, assessed at 2 and 7 days post fertilization, to estimate Pacific oyster larval quality after thawing, and 2) select complementary tools allowing an early and reliable estimation of their quality. Oyster larvae were reared for 25 h after fertilization at 19 degrees C and cryopreserved at early D-stage. Then, thawed larvae were incubated in 2-L beakers. At 2 days post fertilization, the survival rate of thawed Pacific oyster larvae was lower than that of fresh larvae for only one experiment (Experiment 3) among the four identical experiments carried out in this work (Experiments 1-4). By contrast, the survival of thawed larvae, as assessed 7 days after fertilization, was lower than that of fresh larvae for the four experiments. These results confirm that the quality of thawed larvae is lower than that of fresh larvae and that the survival rate, estimated 2 days post fertilization, is not adapted to a reliable estimation of the subsequent development ability of thawed larvae. Then, complementary parameters were tested at 2 days: the movement characteristics (Experiments 1 and 2) and the morphologic features (Experiments 3 and 4) of thawed larvae. Compared to values observed on fresh larvae, the percentage of thawed motile larvae was different for only one experiment (Experiment 2) of the two. Compared to control, a reduced Average Path Velocity (VAP) of larvae (determined at the D-larval stage using a CASA-Computer Assisted Sperm Analysis-system) was observed after thawing for both experiments (Experiments 1 and 2), suggesting the ability of larval movement velocity to assess the decrease of the quality of thawed oyster larvae. Using an ASMA (Automated Sperm Morphology Analysis) device, a lower area of thawed larvae was observed, compared to control and for the two experiments (Experiments 3 and 4). By contrast, the Crofton perimeter of thawed larvae was lower than that of control larvae for only one experiment (Experiment 3) and no significant difference of circularity between fresh and thawed larvae was recorded for Experiments 3 and 4. In conclusion, changes in the movement velocity (assessed by CASA) and in the area (measured by ASMA) of D-larvae allow an early and reliable estimation of the quality of thawed Pacific oyster larvae. (c) 2012 Elsevier Inc. All rights reserved.
PY 2012
PD JUN
SO Theriogenology
SN 0093-691X
PU Elsevier Science Inc
VL 78
IS 2
UT 000306039600025
BP 462
EP 467
DI 10.1016/j.theriogenology.2012.02.014
ID 19934
ER

EF