FN Archimer Export Format
PT J
TI Relevance of Bacteroidales and F-Specific RNA Bacteriophages for Efficient Fecal Contamination Tracking at the Level of a Catchment in France
BT 
AF MAUFFRET, Aourell
   CAPRAIS, Marie-Paule
   GOURMELON, Michele
AS 1:1;2:1;3:1;
FF 1:PDG-RBE-EMP-MICLNR;2:PDG-RBE-EMP-MICLNR;3:PDG-RBE-EMP-MICLNR;
C1 IFREMER, EMP, Microbiol Lab, Plouzane, France.
C2 IFREMER, FRANCE
SI BREST
SE PDG-RBE-EMP-MICLNR
IN WOS Ifremer jusqu'en 2018
IF 3.68
TC 26
UR https://archimer.ifremer.fr/doc/00089/20009/17680.pdf
LA English
DT Article
AB The relevance of three host-associated Bacteroidales markers (HF183, Rum2Bac, and Pig2Bac) and four F-specific RNA bacteriophage genogroups (FRNAPH I to IV) as microbial source tracking markers was assessed at the level of a catchment (Daoulas, France). They were monitored together with fecal indicators (Escherichia coli and enterococci) and chemophysical parameters (rainfall, temperature, salinity, pH, and turbidity) by monthly sampling over 2 years (n = 240 water samples) and one specific sampling following an accidental pig manure spillage (n = 5 samples). During the 2-year regular monitoring, levels of E. coil, enterococci, total F-specific RNA bacteriophages, and the general Bacteroidales marker AllBac were strongly correlated with one another and with Rum2Bac (r = 0.37 to 0.50, P < 0.0001). Their correlations with HF183 and FRNAPH I and II were lower (r = 0.21 to 0.29, P < 0.001 to P < 0.0001), and HF183 and enterococci were associated rather than correlated (Fisher's exact test, P < 0.01). Rum2Bac and FIR 83 enabled 73% of water samples that had >= 2.7 log(10) most probably number (MPN) of E. coli/100 ml to be classified. FRNAPFI I and II enabled 33% of samples at this contamination level to be classified. FRNAPH I and II complemented the water sample classification obtained with the two Bacteroidales markers by an additional 8%. Pig2Bac and FRNAPH III and IV were observed in a small number of samples (n = 0 to 4 of 245). The present study validates Rum2Bac and HF183 as relevant tools to trace fecal contamination originating from ruminant or human waste, respectively, at the level of a whole catchment.
PY 2012
PD AUG
SO Applied And Environmental Microbiology
SN 0099-2240
PU Amer Soc Microbiology
VL 78
IS 15
UT 000306532500015
BP 5143
EP 5152
DI 10.1128/AEM.00315-12
ID 20009
ER

EF