FN Archimer Export Format PT J TI Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags BT AF COUSIN, Xavier DAOUK, Tarek PEAN, Samuel LYPHOUT, Laura SCHWARTZ, Marie-Elise BEGOUT, Marie-Laure AS 1:1,2;2:1,3;3:1,3;4:1;5:1,2,3;6:1; FF 1:;2:;3:;4:PDG-RBE-HGS-LRHLR;5:;6:PDG-RBE-HGS-LRHLR; C1 IFREMER, F-17137 Lhoumeau, France. INRA SCRIBE, F-35042 Rennes, France. UNIV LA ROCHELLE, France C2 IFREMER, FRANCE INRA, FRANCE UNIV LA ROCHELLE, FRANCE SI LA ROCHELLE SE PDG-RBE-BE-LBEX PDG-RBE-HGS-LRHLR PDG-RBE-BE IN WOS Ifremer jusqu'en 2018 copubli-france copubli-p187 copubli-univ-france IF 3.24 TC 32 UR https://archimer.ifremer.fr/doc/00089/20066/17835.pdf LA English DT Article DE ;effects;fish;marking AB Although individual electronic tagging using passive integrated acoustic (PIT) tags is established, it is mainly for fish >60. mm in length and is unsuitable for fish of <30. mm, like zebrafish. We used radio frequency identification (RFID) microtags (1 mm in diameter and 6 mm in length, with a mass of similar to 10 mg) to individually identify juvenile zebrafish (length 16-42 mm, mass 138-776 mg) for the first time, and studied the effects of intracoelomic implantation on fish survival and microtag loss, growth, spawning and exploratory behaviour. After 5.5 months, both high survival (82%) and low microtag loss (11%) were achieved. The smallest surviving fish weighed 178 mg, and success in microtag reading was 73% for the size class 350-450 mg (26 mm). Greater success was achieved when fish were larger at the time of tagging but no negative effects on growth were observed for any size class and some tagged fish spawned. No significant differences in behavioural responses could be detected between tagged fish and untagged controls after 2 months. Overall, the results suggest that the tagging method is highly suitable for fish as small as zebrafish juveniles. We think this method will provide significant advances for researchers of the ever-growing fish model community and more generally for all small-fish users. Tagging is essential when one needs to identify fish (e. g. particular genotypes with no external cue), to run longitudinal monitoring of individual biological traits (e. g. growth) or to repeat assays with the same individual at discrete points in time (e. g. behaviour studies). Such a method will find applications in physiology, genetics, behaviour and (eco)toxicology fields. PY 2012 PD AUG SO Journal Of Experimental Biology SN 0022-0949 PU Company Of Biologists Ltd VL 215 IS 16 UT 000306755700005 BP 2729 EP 2734 DI 10.1242/jeb.071829 ID 20066 ER EF