FN Archimer Export Format
PT J
TI Bacterial problems associated with scallop Pecten maximus larval culture
BT 
AF NICOLAS, Jean-Louis
   CORRE, S
   GAUTHIER, Gilles
   ROBERT, Rene
   ANSQUER, Dominique
AS 1:;2:;3:;4:;5:;
FF 1:;2:;3:;4:;5:;
C1 MICROMER,F-29200 BREST,FRANCE.
   CNRS,F-06230 VILLEFRANCHE MER,FRANCE.
   UNIV PARIS 06,STN ZOOL,F-06230 VILLEFRANCHE MER,FRANCE.
C2 MICROMER,F-29200 BREST,FRANCE.
   CNRS,F-06230 VILLEFRANCHE MER,FRANCE.
   UNIV PARIS 06,STN ZOOL,F-06230 VILLEFRANCHE MER,FRANCE.
SI BREST
   ARGENTON
   SAINT VINCENT
IF 1.556
TC 137
UR https://archimer.ifremer.fr/doc/00090/20077/17716.pdf
LA English
DT Article
DE ;larvae;bivalve;vibrio;scallop
AB Scallop Pecten maximus larvae have been cultured at the Argenton and Tinduff (Brittany, France) hatcheries with antibiotic treatment (chloramphenicol at 8 ppm) for 15 yr. Without treatment, outbreak of disease has normally occurred between Day 12 and Day 19 or sometimes earlier. A bacteriological study of larvae reared with and without antibiotic was performed over a 4 yr period. Among the collected strains, 2 clusters (C and F) of vibrios were present at high densities only in larvae cultured without treatment. One cluster (C) was routinely isolated over the 4 yr of study, while the other (F) was collected only in the third year. Their virulence with respect to scallop larvae and their lack of infectivity with respect to oyster larvae were demonstrated in an exposure experiment. The vibrio F strain tended to lose its virulence after 5 subcultures, whereas the vibrio C strain retained the ability to kill scallop larvae in experimental infections. Three other vibrios isolated in moribund oyster larvae caused mortality in oyster larvae but not in scallop larvae. Different methods were used to determine the taxonomic position of these virulent bacteria. The phenotypic traits of bacterial isolates were determined with the Biolog GN microplate, the API 20E system and the reference method. Patterns of cytoplasmic proteins were identified by electrophoresis in SDS-PAGE. These different methods consistently confirmed the existence of 2 vibrio species pathogenic to scallop larvae. Affiliation of cluster F with Vibrio splendidus was assessed by Biolog tests and by analysis of 16S rRNA sequences. One pathogenic bacteria of oyster larvae was also very close to this second cluster, whereas the 2 others from moribund oyster larvae and cluster C may constitute 2 different species.
PY 1996
PD OCT
SO Diseases Of Aquatic Organisms
SN 0177-5103
PU Inter-research
VL 27
IS 1
UT A1996VX34800010
BP 67
EP 76
DI 10.3354/dao027067
ID 20077
ER

EF