FN Archimer Export Format
PT J
TI One Perkinsus species may hide another: characterization of Perkinsus species present in clam production areas of France
BT 
AF ARZUL, Isabelle
   CHOLLET, Bruno
   MICHEL, J
   ROBERT MILL, Hugh
   GARCIA, Celine
   JOLY, Jean-Pierre
   FRANCOIS, Cyrille
   MIOSSEC, Laurence
AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;8:2;
FF 1:PDG-RBE-SG2M-LGPMM;2:PDG-RBE-SG2M-LGPMM;3:;4:;5:PDG-RBE-SG2M-LGPMM;6:PDG-RBE-SG2M-LGPMM;7:PDG-RBE-SG2M-LGPMM;8:PDG-ODE-DYNECO-VIGIES;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   IFREMER, Dept Data Dev Integrated Management & Survey, F-44311 Nantes 03, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   NANTES
SE PDG-RBE-SG2M-LGPMM
   PDG-ODE-DYNECO-VIGIES
IN WOS Ifremer jusqu'en 2018
IF 2.36
TC 31
UR https://archimer.ifremer.fr/doc/00094/20542/18205.pdf
LA English
DT Article
DE ;clam parasites;Perkinsus chesapeaki;Perkinsus olseni;Ruditapes philippinarum;Ruditapes decussatus;ribosomal RNA;actin;ISH
AB Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in Bonne Anse in Charente Maritime, on the Atlantic coast. Clonal cultures from both detected species were produced in order to describe and compare in vitro stages. Differences in size between both Perkinsus spp. were noticed especially for schizonts and zoosporangia. Lastly, in situ hybridization tests allowed confirmation of the presence of both species in the same R. decussatus population and even in same clams. This is the first detection of P. chesapeaki in Ruditapes species and outside North America, which questions its introduction into Europe.
PY 2012
PD NOV
SO Parasitology
SN 0031-1820
PU Cambridge Univ Press
VL 139
IS 13
UT 000310549100009
BP 1757
EP 1771
DI 10.1017/S0031182012001047
ID 20542
ER

EF