Development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the DNA polymerase gene

Type Article
Date 2012-10
Language English
Author(s) Chen M.H.1, 3, Kuo S. T.2, Renault Tristan4, Friedman C. S.5, Chang P. H.1
Affiliation(s) 1 : Natl Taiwan Univ, Sch Vet Med, Inst Vet Med, Taipei 10764, Taiwan.
2 : Natl Inst Anim Hlth, Tansui, Taiwan.
3 : Tzu Chi Coll Technol, Hualien, Taiwan.
4 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
5 : Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA.
Source Journal Of Virological Methods (0166-0934) (Elsevier Science Bv), 2012-10 , Vol. 185 , N. 1 , P. 1-6
DOI 10.1016/j.jviromet.2012.03.024
WOS© Times Cited 1
Keyword(s) Herpes virus, Abalone, Haliotis diversicolor supertexta, DNA polymerase, Polymerase chain reaction
Abstract A 5781-base pair (bp) fragment of genomic DNA from the Taiwanese abalone herpesvirus was obtained and showed 99% (5767/5779) homology in the nucleotide sequence and 99% (1923/1926) in the amino acid sequence with the DNA polymerase gene of the abalone herpesvirus strain Victoria/AUS/2007. Homology of the amino acid sequence with the DNA polymerase of ostreid herpesvirus 1 was 30% (563/1856). In this study, a PCR-based procedure for detecting herpesvirus infection of abalone, Haliotis diversicolor supertexta, in Taiwan was developed. The method employed primer sets targeting the viral DNA polymerase gene, and was able to amplify DNA fragments of the expected size from infected samples. Primer sets of 40f and 146r were designed for amplification of an expected PCR product of 606 bp. Combining the new PCR protocol with histopathology, this assay can serve as a reliable diagnostic for herpesvirus infections in abalone. (C) 2012 Elsevier B.V. All rights reserved.
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