FN Archimer Export Format
PT J
TI Development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the DNA polymerase gene
BT 
AF CHEN, M.H.
   KUO, S. T.
   RENAULT, Tristan
   FRIEDMAN, C. S.
   CHANG, P. H.
AS 1:1,3;2:2;3:4;4:5;5:1;
FF 1:;2:;3:PDG-RBE-AGSAE-LGP;4:;5:;
C1 Natl Taiwan Univ, Sch Vet Med, Inst Vet Med, Taipei 10764, Taiwan.
   Natl Inst Anim Hlth, Tansui, Taiwan.
   Tzu Chi Coll Technol, Hualien, Taiwan.
   IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA.
C2 UNIV NATL TAIWAN NTU, TAIWAN
   ANIM HLTH RES INST AHRI, TAIWAN
   UNIV TZU CHI TCUST, TAIWAN
   IFREMER, FRANCE
   UNIV WASHINGTON, USA
SI LA TREMBLADE
SE PDG-RBE-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
IF 1.9
TC 3
UR https://archimer.ifremer.fr/doc/00098/20952/19675.pdf
LA English
DT Article
DE ;Herpes virus;Abalone;Haliotis diversicolor supertexta;DNA polymerase;Polymerase chain reaction
AB A 5781-base pair (bp) fragment of genomic DNA from the Taiwanese abalone herpesvirus was obtained and showed 99% (5767/5779) homology in the nucleotide sequence and 99% (1923/1926) in the amino acid sequence with the DNA polymerase gene of the abalone herpesvirus strain Victoria/AUS/2007. Homology of the amino acid sequence with the DNA polymerase of ostreid herpesvirus 1 was 30% (563/1856). In this study, a PCR-based procedure for detecting herpesvirus infection of abalone, Haliotis diversicolor supertexta, in Taiwan was developed. The method employed primer sets targeting the viral DNA polymerase gene, and was able to amplify DNA fragments of the expected size from infected samples. Primer sets of 40f and 146r were designed for amplification of an expected PCR product of 606 bp. Combining the new PCR protocol with histopathology, this assay can serve as a reliable diagnostic for herpesvirus infections in abalone. (C) 2012 Elsevier B.V. All rights reserved.
PY 2012
PD OCT
SO Journal Of Virological Methods
SN 0166-0934
PU Elsevier Science Bv
VL 185
IS 1
UT 000307615400001
BP 1
EP 6
DI 10.1016/j.jviromet.2012.03.024
ID 20952
ER

EF