Spirolide uptake and detoxification by Crassostrea gigas exposed to the toxic dinoflagellate Alexandrium ostenfeldii

Type Article
Date 2012-08
Language English
Author(s) Medhioub Walid1, 2, 3, Lassus Patrick1, Truquet Philippe1, Bardouil Michele1, Amzil Zouher1, Sechet VeroniqueORCID1, Sibat ManoellaORCID1, Soudant Philippe3
Affiliation(s) 1 : IFREMER, Lab Phycotoxines, F-44311 Nantes 3, France.
2 : INSTM, Lab Aquaculture, Monastir 5000, Tunisia.
3 : Univ Bretagne Occidentale, IUEM, LEMAR CNRS UMR 6539, F-29280 Plouzane, France.
Source Aquaculture (0044-8486) (Elsevier Science Bv), 2012-08 , Vol. 358-359 , P. 108-115
DOI 10.1016/j.aquaculture.2012.06.023
WOS© Times Cited 13
Keyword(s) Spirolides, Alexandrium ostenfeldii, Contamination, Depuration, Crassostrea gigas, Inflammatory responses
Abstract Oysters (Crassostrea gigas) were exposed 4 days to cultures of the toxic dinoflagellate Alexandrium ostenfeldii (strain CCMP1773) that produces spirolides belonging to fast acting toxins (FAT) and let depurate for 7 days. During depuration, oysters were either fed the non-toxic algae Isochrysis galbana Tahitian clone (T. Iso) or starved. The objectives of this experiment were to evaluate (i) spirolide uptake and depuration by oysters (ii) spirolide effects on oysters and (iii) oyster recovery according to food supply during depuration. A. ostenfeldii cells were filtered and ingested by oysters while faeces contained numerous intact cells of the toxic diet. This suggested that ingested cells were not totally digested by oysters. Contents of spirolides (SPX) in digestive gland and remaining tissues during contamination and detoxification periods were measured by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Four different SPX analogues (13,19-didesMeC, 13-desMeC, 13-desMeD and trace of SPX-D) were detected. The 13,19-didesMeC-SPX dominated in both digestive gland and remaining tissues. After four days exposure, digestive gland (DG) contained 83% of the total initial spirolide concentration, whereas remaining tissues contained only 17%. During detoxification, spirolide content in DG was lower in fed than in unfed oysters but similar in remaining tissues. Exposure to A. ostenfeldii resulted in an inflammatory response consisting of hemocyte infiltration and diapedesis into the intestinal tract of the oysters. Percentage of active digestive tubules in oysters fed A. ostenfeldii was significantly lower than in control (prior exposition) oysters (36% and 61%, respectively). At the end of the detoxification period, there was a significant difference in the percentage of active digestive tubules (P<0.001) between fed and unfed oysters. When oysters were fed T-Iso following the A. ostenfeldii exposure, 80% of digestive tubules were active, thus revealing a rapid recovery after toxic algae exposure. Overall, both spirolide detoxification and recovery from their toxic effects are almost complete within 7 days after exposure to spirolide producers. Such informationmay help to resume faster oyster sales after toxic events involving FAT implying thus more frequent chemical analysis. (C) 2012 Elsevier B. V. All rights reserved.
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