FN Archimer Export Format
PT J
TI Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri)
BT 
AF OVANDO, Fernanda
   GIMPEL, Carla
   CARDENAS, Constanza
   MACHADO CUNHA DA SILVA, Jose Roberto
   DE LORGERIL, Julien
   GONZALEZ, MArcelo
AS 1:1;2:1;3:2;4:3;5:4;6:1;
FF 1:;2:;3:;4:;5:PDG-RBE-BOME-LALR;6:;
C1 Inst Antartico Chileno, Lab Biorrecursos Antarticos, Punta Arenas, Chile.
   Pontificia Univ Catolica Valparaiso, Nucleo Biotecnol Curauma, Valparaiso, Chile.
   Univ Sao Paulo, CEBIMar, Inst Ciencias Biomed 1, Dept Histol & Embriol, BR-05508 Sao Paulo, Brazil.
   IFREMER, Univ Montpellier 2, CNRS, IRD,UMR Ecosyst Lagunaires 5119, F-34095 Montpellier 5, France.
C2 INST ANTARTICO CHILENO, CHILE
   UNIV PONTIFICIA CATOLICA VALPARAISO, CHILE
   UNIV SAO PAULO, BRAZIL
   IFREMER, FRANCE
SI MONTPELLIER
SE PDG-RBE-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
   copubli-sud
IF 0.87
TC 19
UR https://archimer.ifremer.fr/doc/00098/20955/18572.pdf
LA English
DT Article
DE ;Antarctica;sea urchin;Sterechinus neumayeri;coelomocytes;gene expression;AIF-1
AB We have cloned and characterized for the first time an allograft inflammatory factor 1 (Sn-AIF-1) from the Antarctic sea urchin. We report the cloning of Sn-AIF-1 cDNA and the characterization of its expression in coelomocytes after a bacterial challenge. The cDNA Sn-AIF-1 has a size of 608 bp and encodes a polypeptide of 151 aa. The deduced amino acid sequence has a putative size of 17.430 Da, an isoelectric point of 4.92, and shows 2 elongation factor handlike motifs that normally bind calcium ions. BLAST analysis revealed close matches with other known AIF-1. The deduced amino acid sequence of Sn-AIF-1 showed high homology with AIF-1 in vertebrates such as fish, mice, and humans; and in the case of invertebrates, the major degree of identity (55%) was with a predicted sequence of the purple sea urchin AIF-1, and 52% corresponded to a sponge. Expression of Sn-AIF-1 mRNA was analyzed by qPCR. Sn-AIF-1 mRNA expression was measured from coelomocytes after a bacterial challenge using RT-PCR and revealed that the gene was upregulated after 24 h. Sn-AIF-1 could participate in the inflammatory response, particularly in the activation of coelomocytes and their survival.
PY 2012
PD AUG
SO Journal Of Shellfish Research
SN 0730-8000
PU Natl Shellfisheries Assoc
VL 31
IS 3
UT 000308254800036
BP 875
EP 883
DI 10.2983/035.031.0336
ID 20955
ER

EF