DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant?
|Author(s)||Lazerges M.1, 2, Perrot H.1, 2, Rabehagasoa N.3, Compere Chantal3, Dreanno Catherine3, Mucio Pedroso M.4, Faria R. C.4, Bueno P. R.5|
|Affiliation(s)||1 : CNRS, UPR 15, Lab Interfaces & Syst Electrochim, F-75252 Paris, France.
2 : Univ Paris 06, LISE, F-75252 Paris, France.
3 : IFREMER, Ctr Brest, Serv Interfaces & Capteurs, ZI Pointe Diable, F-29280 Plouzane, France.
4 : Univ Fed Sao Carlos, Dept Quim, BR-13560905 Sao Paulo, Brazil.
5 : Univ Estadual Paulista, Dept Fisicoquim, Inst Quim, BR-14800900 Sao Paulo, Brazil.
|Source||Sensors And Actuators B-chemical (0925-4005) (Elsevier Science Sa), 2012-08 , Vol. 171 , P. 522-527|
|WOS© Times Cited||5|
|Keyword(s)||Biosensors, DNA, Hybridization kinetics, Quartz crystal microbalance|
|Abstract||The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and increases with DNA target length. It was established that k(2) (s(-1)), relevant to irreversible formation of a stable duplex, varies in an opposite way to K-1 with DNA target length. (C) 2012 Published by Elsevier B.V.|